Multivalent forms of the ribonuclease H1 hybrid binding domain are high‐affinity binders of RNA–DNA hybrids
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DOI: 10.1002/1873-3468.14541
Abstract: The hybrid binding domain (HBD) is a conserved fold present in ribonucleases H1 that selectively recognizes RNA–DNA hybrids, which are structures present in cellular R‐loops and participate in diverse biological processes. We engineered multivalent HBD… read more here.
Keywords: rna dna; hybrid binding; high affinity; affinity ... See more keywords
Optimizing hybrid LC-MS/MS binding conditions is critical: impact of biotransformation on quantification of trastuzumab.
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DOI: 10.4155/bio-2018-0196
Abstract: BACKGROUND Hybrid ligand-binding (LB) LC-MS/MS protein quantitative assays involve a LB step for analyte enrichment that has less stringent requirements than the conventional LB assays. RESULTS Herceptin™(trastuzumab) binding to HER2 extracellular domain was evaluated using… read more here.
Keywords: binding conditions; optimizing hybrid; hybrid binding; conditions critical ... See more keywords