An examination of critical parameters in hybridization‐based epigenotyping using magnetic microparticles
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Progress"
DOI: 10.1002/btpr.2644
Abstract: Gene‐specific promoter methylation is involved in gene silencing and is an important cancer biomarker. Cancer‐specific methylation patterns have been observed and clinically validated for numerous gene promoters, but the knowledge gleaned from this large body… read more here.
Keywords: methylation; hybridization; magnetic microparticles; based epigenotyping ... See more keywords
Nucleic Acid Hybridization-Based Noise Suppression for Ultraselective Multiplexed Amplification of Mutant Variants.
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DOI: 10.1002/smll.202006370
Abstract: The analysis of mutant nucleic acid (NA) variants can provide crucial clinical and biological insights for many diseases. Yet, existing analysis techniques are generally constrained by nonspecific "noise" signals from excessive wildtype background sequences, especially… read more here.
Keywords: hybridization; ultraselective multiplexed; mutant variants; hybridization based ... See more keywords
Importance of probe design for bioanalysis of oligonucleotides using hybridization-based LC-fluorescence assays.
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DOI: 10.4155/bio-2019-0154
Abstract: The importance of the length and/or structure of fluorescently labeled PNA (peptide nucleic acid) probes for quantitative determination of oligodeoxynucleotides (ODNs) is demonstrated in human plasma using hybridization-based LC-fluorescence assays. The length of the PNA… read more here.
Keywords: using hybridization; importance; based fluorescence; fluorescence assays ... See more keywords