Hybridization Chain Reaction Design and Biosensor Implementation.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8582-1_8
Abstract: DNA biosensors could overcome some of the common drawbacks of lab-based techniques for nucleic acids detection for diagnostics purposes. One of the main impediments for such applications of DNA biosensors is their lack of sensitivity:… read more here.
Keywords: hybridization chain; chain reaction; dna biosensors;
Magnetic beads-based DNA hybridization chain reaction amplification and DNAzyme recognition for colorimetric detection of uranyl ion in seafood.
Sign Up to like & getrecommendations! Published in 2017 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2016.12.021
Abstract: A novel colorimetric biosensor, which employs DNAzyme-functionalized magnetic beads (MBs) as recognition probe, enzyme-assisted catalytic oxidation of TMB (3,3',5,5'-tetramethylbenzidine sulfate) as signal and DNA hybridization chain reaction as amplification strategy, has been developed for detecting… read more here.
Keywords: dna hybridization; hybridization chain; chain reaction;
A signal-flexible gene diagnostic strategy coupling loop-mediated isothermal amplification with hybridization chain reaction
Sign Up to like & getrecommendations! Published in 2019 at "Analytica Chimica Acta"
DOI: 10.1016/j.aca.2019.06.048
Abstract: Abstract Recent study proves that the combination of loop mediated isothermal nucleic acid amplification (LAMP) with one-step strand displacement (OSD) is of great help to improve the sequence specificity during genetic detection. However, because OSD… read more here.
Keywords: loop mediated; mediated isothermal; hybridization chain; chain reaction ... See more keywords
A ratiometric electrochemical assay for human 8-oxoguanine DNA glycosylase amplified by hybridization chain reaction
Sign Up to like & getrecommendations! Published in 2019 at "Electrochemistry Communications"
DOI: 10.1016/j.elecom.2019.05.006
Abstract: Abstract Human 8-oxoguanine DNA glycosylase 1 (hOGG1) is an important DNA repair enzyme, which is closely related to the occurrence and development of certain diseases. Ultrasensitive and accurate detection of hOGG1 is significant for fundamental… read more here.
Keywords: oxoguanine dna; dna; dna glycosylase; ratiometric electrochemical ... See more keywords
Highly sensitive antibody-aptamer sensor for vascular endothelial growth factor based on hybridization chain reaction and pH meter/indicator.
Sign Up to like & getrecommendations! Published in 2017 at "Talanta"
DOI: 10.1016/j.talanta.2017.04.073
Abstract: Vascular endothelial growth factor (VEGF) is a crucial signaling protein for the tumor growth and metastasis, which is also acted as the biomarkers for various diseases. In this research, we fabricate an aptamer-antibody sensor for… read more here.
Keywords: hybridization chain; vascular endothelial; endothelial growth; antibody ... See more keywords
Proximity hybridization triggered hybridization chain reaction for label-free electrochemical homogeneous aptasensors.
Sign Up to like & getrecommendations! Published in 2021 at "Talanta"
DOI: 10.1016/j.talanta.2020.122058
Abstract: A label-free homogeneous electrochemical aptasensor was developed for detection of thrombin based on proximity hybridization triggered hybridization chain reaction induced G-quadruplex formation. Thrombin promoted the formation of a complex via the proximity hybridization of the… read more here.
Keywords: hybridization chain; proximity hybridization; hybridization; chain reaction ... See more keywords
Hybridization chain reaction and its applications in biosensing.
Sign Up to like & getrecommendations! Published in 2021 at "Talanta"
DOI: 10.1016/j.talanta.2021.122637
Abstract: To pursue the sensitive and efficient detection of informative biomolecules for bioanalysis and disease diagnosis, a series of signal amplification techniques have been put forward. Among them, hybridization chain reaction (HCR) is an isothermal and… read more here.
Keywords: chain reaction; hybridization chain; reaction; hcr ... See more keywords
Light-Controlled Recruitable Hybridization Chain Reaction on Exosome Vehicles for Highly Sensitive MicroRNA Imaging in Living Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c00974
Abstract: Sensitive imaging of intracellular microRNA (miRNA) in living cells is of great significance. Isothermal hybridization chain reaction (HCR)-based methods, although have been widely used to monitor intracellular low-abundance miRNA, are still subjected to the challenges… read more here.
Keywords: hybridization chain; light controlled; chain reaction; controlled recruitable ... See more keywords
A Reciprocal-Amplifiable Fluorescence Sensing Platform via Replicated Hybridization Chain Reaction for Hosting Concatenated Multi-Ag Nanoclusters as Signal Reporter.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03782
Abstract: Exploring the replication of hybridization chain reaction HCR (rHCR) for reciprocal amplification is intriguing in biosensing and bioanalysis. Herein, we develop a rHCR-based fluorescence platform that is manipulated by the combination of a specific DNA… read more here.
Keywords: hybridization chain; chain reaction; rhcr; hybridization ... See more keywords
Ultrasensitive Hybridization Chain Reaction-Assisted Multisite Exonuclease III Amplification Strategy Combined with a Direct Quantitative Fluorescence Lateral Flow Technique for Multiple Bacterial 16S rRNA Detection.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c00270
Abstract: Accurate and in-time detection of bacteria conduces to preventing their rapid spread around the environment, while a nucleic acid test (NAT) is a powerful tool for early diagnosis of pathogens. Herein, we propose a hybridization… read more here.
Keywords: hybridization chain; 16s rrna; amplification; iii ... See more keywords
DNA Nanofirecrackers Assembled through Hybridization Chain Reaction for Ultrasensitive SERS Immunoassay of Prostate Specific Antigen.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b05648
Abstract: Isothermal nucleic acid amplification technology has widely adopted for analytical chemistry with the purpose for sensitivity improvement. Herein we present an ultrasensitive concatenated hybridization chain reaction (C-HCR) based surface-enhanced Raman scattering (SERS) immunoassay by forming… read more here.
Keywords: hybridization chain; sers immunoassay; chemistry; dna nanofirecrackers ... See more keywords