Hybridoma Screening by Antibody Capture: Detection of Intracellular Binding by Immunofluorescence.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot103101
Abstract: Hybridoma screening by immunofluorescence stainings of whole cells can be adapted to screen for antibodies to internal proteins by permeabilizing the cells before applying the hybridoma supernatants. In this protocol, cells are attached to a… read more here.
Keywords: capture detection; hybridoma screening; screening antibody; immunofluorescence ... See more keywords
Abstract 5774: Novel cell-based high-throughput hybridoma screening method using the Celigo image cytometer for antibody discovery
Sign Up to like & getrecommendations! Published in 2018 at "Cancer Research"
DOI: 10.1158/1538-7445.am2018-5774
Abstract: Hybridoma screening is a highly important process for antibody discovery, which can identify potential clones from hundreds to thousands of hybridoma cultures against the desired target antigen. Traditional screening methods using ELISA and flow cytometry… read more here.
Keywords: image; antibody; hybridoma; image cytometer ... See more keywords