Hybridoma Screening by Antibody Capture: Enzyme-Linked Detection (Indirect ELISA) in Polyvinyl Chloride Wells.
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DOI: 10.1101/pdb.prot103044
Abstract: In this antibody capture assay for hybridoma screening, the antigen is immobilized on a solid substrate (the surface of the wells in a polyvinyl chloride [PVC] microtiter plate), and antibodies in the hybridoma tissue culture… read more here.
Keywords: hybridoma screening; polyvinyl chloride; antibody; antibody capture ... See more keywords
Hybridoma Screening by Antibody Capture: Flow Cytometry/FACS with Permeabilized Cells to Detect Intracellular Binding.
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DOI: 10.1101/pdb.prot103085
Abstract: Flow cytometry or fluorescence-activated cell sorting (FACS) can be used to identify hybridomas secreting monoclonal antibodies to internal cellular proteins, but the cells must be permeabilized before the hybridoma supernatants are applied. In using this… read more here.
Keywords: hybridoma screening; flow cytometry; screening antibody; cytometry ... See more keywords
Hybridoma Screening by Antibody Capture: Detection of Cell-Surface Binding by Immunofluorescence.
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DOI: 10.1101/pdb.prot103093
Abstract: If the antigen of interest is a cell-surface protein, immunofluorescence can be used to identify hybridomas secreting monoclonal antibodies to these proteins. They can be stained on microscope chamber slides, flat-bottomed cell culture plates (96,… read more here.
Keywords: cell; hybridoma screening; cell surface; screening antibody ... See more keywords
Hybridoma Screening by Antibody Capture: Detection of Intracellular Binding by Immunofluorescence.
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DOI: 10.1101/pdb.prot103101
Abstract: Hybridoma screening by immunofluorescence stainings of whole cells can be adapted to screen for antibodies to internal proteins by permeabilizing the cells before applying the hybridoma supernatants. In this protocol, cells are attached to a… read more here.
Keywords: capture detection; hybridoma screening; screening antibody; immunofluorescence ... See more keywords
Hybridoma Screening by Antigen Capture: Immunoprecipitation.
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DOI: 10.1101/pdb.prot103143
Abstract: Immunoprecipitation is rarely used for screening hybridoma fusions because the assays are tedious and time-consuming. However, it can be useful when working with complex antigens because the precipitated antigen is normally detected after sodium dodecyl… read more here.
Keywords: antigen capture; hybridoma screening; capture immunoprecipitation; screening antigen ... See more keywords