Epitope Screening Using Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS): An Accelerated Workflow for Evaluation of Lead Monoclonal Antibodies.
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology journal"
DOI: 10.1002/biot.202100358
Abstract: BACKGROUND Epitope mapping is an increasingly important aspect of biotherapeutic and vaccine development. Recent advances in therapeutic antibody design and production have enabled candidate mAbs to be identified at a rapidly increasing rate, resulting in… read more here.
Keywords: mass spectrometry; exchange mass; epitope screening; hydrogen deuterium ... See more keywords
Mapping the Interactions of Selective Biochemical Probes of Antibody Conformation by Hydrogen–Deuterium Exchange Mass Spectrometry
Sign Up to like & getrecommendations! Published in 2017 at "ChemBioChem"
DOI: 10.1002/cbic.201600670
Abstract: Protein‐based pharmaceuticals represent the fastest growing group of drugs in development in the pharmaceutical industry. One of the major challenges in the discovery, development, and distribution of biopharmaceuticals is the assessment of changes in their… read more here.
Keywords: mass spectrometry; exchange mass; antibody; hydrogen deuterium ... See more keywords
Rapid antibody conformational screening by matrix assisted laser desorption ionization hydrogen-deuterium exchange mass spectrometry.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of separation science"
DOI: 10.1002/jssc.202100986
Abstract: Recent advances in the field of cancer biology have accelerated the discovery and development of novel biopharmaceuticals. At the forefront of these drug development efforts are high-throughput screening, compressed timelines and limited sample quantities, all… read more here.
Keywords: assisted laser; laser desorption; desorption ionization; hydrogen deuterium ... See more keywords
Bridging protein structure, dynamics, and function using hydrogen/deuterium‐exchange mass spectrometry
Sign Up to like & getrecommendations! Published in 2019 at "Protein Science"
DOI: 10.1002/pro.3790
Abstract: Much of our understanding of protein structure and mechanistic function has been derived from static high‐resolution structures. As structural biology has continued to evolve it has become clear that high‐resolution structures alone are unable to… read more here.
Keywords: protein; protein structure; hydrogen deuterium; function ... See more keywords
Studying Protein-DNA Interactions by Hydrogen/Deuterium Exchange Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1126-5_11
Abstract: Protein hydrogen/deuterium exchange (HDX) coupled to mass spectrometry (MS) can be used to study interactions of proteins with various ligands, to describe the effects of mutations, or to reveal structural responses of proteins to different… read more here.
Keywords: protein; mass spectrometry; dna; protein dna ... See more keywords
Epitope mapping of antibodies in C-reactive protein assay kits by hydrogen-deuterium exchange mass spectrometry explains differential results across kits
Sign Up to like & getrecommendations! Published in 2022 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-022-04029-z
Abstract: C-Reactive protein (CRP) is an important marker for in vitro diagnosis (IVD) of inflammation. However, CRP immunoturbidimetric kits from different manufacturers exhibit inconsistency in evaluation, making clinical diagnosis challenging. The use of immunological methods in… read more here.
Keywords: reactive protein; epitope mapping; exchange mass; hydrogen deuterium ... See more keywords
Hydrogen/Deuterium Exchange Behavior During Denaturing/Refolding Processes Determined in Tetragonal Hen Egg-White Lysozyme Crystals
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Biotechnology"
DOI: 10.1007/s12033-022-00447-7
Abstract: The hydrogen/deuterium (H/D) exchange of main-chain amide hydrogens in the protein that denatured and refolded in deuterated solvent is considered to contain the traces of hydrogen bond cleavages or the exposure to solvent of the… read more here.
Keywords: hen egg; denaturing refolding; refolding processes; hydrogen deuterium ... See more keywords
Solute hydrogen and deuterium observed at the near atomic scale in high-strength steel
Sign Up to like & getrecommendations! Published in 2020 at "Acta Materialia"
DOI: 10.1016/j.actamat.2020.02.004
Abstract: Abstract Observing solute hydrogen (H) in matter is a formidable challenge, yet, enabling quantitative imaging of H at the atomic-scale is critical to understand its deleterious influence on the mechanical strength of many metallic alloys… read more here.
Keywords: strength; hydrogen; solute hydrogen; hydrogen deuterium ... See more keywords
Improving accuracy of Penning gauge spectroscopy for the determination of hydrogen isotope H/D ratios
Sign Up to like & getrecommendations! Published in 2017 at "Fusion Engineering and Design"
DOI: 10.1016/j.fusengdes.2017.03.092
Abstract: Abstract Atomic spectral lines emitted from a Penning discharge are often used to quantify partial pressures and isotopes ratios in gases. To identify the potential of this method for thermal desorption studies, the hydrogen emission… read more here.
Keywords: hydrogen; determination; hydrogen deuterium; penning gauge ... See more keywords
In-situ neutron diffraction investigation of Mg2FeH6 dehydrogenation
Sign Up to like & getrecommendations! Published in 2017 at "International Journal of Hydrogen Energy"
DOI: 10.1016/j.ijhydene.2016.11.157
Abstract: Abstract In this paper we present an in-situ neutron diffraction experiment on the dehydrogenation of Mg 2 FeH 6 . Stoichiometric mixtures of 2Mg + Fe were ball milled in a hydrogen environment. After desorption, the samples… read more here.
Keywords: neutron diffraction; situ neutron; dehydrogenation; hydrogen deuterium ... See more keywords
Neutron scattering study of tantalum monohydride and monodeuteride
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Hydrogen Energy"
DOI: 10.1016/j.ijhydene.2021.03.149
Abstract: Abstract Powder samples of TaH0.89 and TaD0.96 are synthesized under a hydrogen (deuterium) pressure of 2.8 GPa and a temperature of 250 °C, then quenched to the liquid nitrogen temperature, recovered to ambient pressure and studied by… read more here.
Keywords: neutron scattering; hydrogen; study; hydrogen deuterium ... See more keywords