Non-equilibrium hydrogen exchange for determination of H-bond strength and water accessibility in solid proteins
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Biomolecular NMR"
DOI: 10.1007/s10858-017-0110-0
Abstract: We demonstrate measurement of non-equilibrium backbone amide hydrogen–deuterium exchange rates (HDX) for solid proteins. The target of this study are the slowly exchanging residues in solid samples, which are associated with stable secondary-structural elements of… read more here.
Keywords: solid proteins; hydrogen; hydrogen exchange; non equilibrium ... See more keywords
Reference Parameters for Protein Hydrogen Exchange Rates
Sign Up to like & getrecommendations! Published in 2018 at "Journal of The American Society for Mass Spectrometry"
DOI: 10.1007/s13361-018-2021-z
Abstract: AbstractThe analysis of many hydrogen exchange (HX) experiments depends on knowledge of exchange rates expected for the unstructured protein under the same conditions. We present here some minor adjustments to previously calibrated values and a… read more here.
Keywords: protein hydrogen; parameters protein; hydrogen exchange; exchange rates ... See more keywords
NMR Analysis of Amide Hydrogen Exchange Rates in a Pentapeptide-Repeat Protein from A. thaliana.
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical journal"
DOI: 10.1016/j.bpj.2017.04.016
Abstract: At2g44920 from Arabidopsis thaliana is a pentapeptide-repeat protein (PRP) composed of 25 repeats capped by N- and C-terminal α-helices. PRP structures are dominated by four-sided right-handed β-helices typically consisting of mixtures of type II and type… read more here.
Keywords: amide hydrogen; pentapeptide repeat; hydrogen exchange; exchange rates ... See more keywords
Propane activation on Zn-modified zeolite. The effect of the nature of Zn-species on the mechanism of H/D hydrogen exchange of the alkane with Brønsted acid sites
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Catalysis"
DOI: 10.1016/j.jcat.2019.09.006
Abstract: Abstract Zn-modified zeolites exhibit high activity for the aromatization of C2+ alkanes. To understand the effect of Zn-species of different nature on propane molecules activation and transformation, the H/D hydrogen exchange of zeolite Bronsted acid… read more here.
Keywords: hydrogen exchange; effect; acid sites; propane ... See more keywords
Structural Characterization of the Cooperativity of Unfolding of a Heterodimeric Protein Using Hydrogen Exchange-Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2021.167268
Abstract: Little is known about how the sequence of structural changes in one chain of a heterodimeric protein is coupled to those in the other chain during protein folding and unfolding reactions, and whether individual secondary… read more here.
Keywords: protein; chain; heterodimeric protein; exchange mass ... See more keywords
Statistical Equivalence Testing of Higher-Order Protein Structures with Differential Hydrogen Exchange-Mass Spectrometry (HX-MS).
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c05279
Abstract: Hydrogen exchange-mass spectrometry (HX-MS) is widely recognized for its potential utility for establishing the equivalence of the higher-order structures of proteins, particularly in comparability and similarity contexts. However, recent progress in the statistical analysis of… read more here.
Keywords: exchange mass; equivalence; equivalence testing; higher order ... See more keywords
Empirical Method To Accurately Determine Peptide-Averaged Protection Factors from Hydrogen Exchange MS Data.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.6b03908
Abstract: Amide hydrogen exchange experiments measured by mass spectrometry have become commonplace to study protein structural dynamics; however, the underdetermined nature of these measurements render extraction of exchange rates unreliable at the level of individual peptides.… read more here.
Keywords: protection; method accurately; method; hydrogen exchange ... See more keywords
Rate of Asparagine Deamidation in a Monoclonal Antibody Correlating with Hydrogen Exchange Rate at Adjacent Downstream Residues.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.6b04158
Abstract: Antibodies are an important class of drugs, comprising more than half of all new FDA approvals. Therapeutic antibodies must be chemically stable both in storage and in vivo, following administration to patients. Deamidation is a… read more here.
Keywords: rate adjacent; hydrogen exchange; rate; deamidation ... See more keywords
A Structural Variant Approach for Establishing a Detection Limit in Differential Hydrogen Exchange-Mass Spectrometry Measurements.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b01326
Abstract: Hydrogen exchange-mass spectrometry (HX-MS) is widely promoted for its ability to detect subtle perturbations in protein structure, but such perturbations will result in small differences in HX. However, the detection limit of HX-MS has not… read more here.
Keywords: detection; structural variant; hydrogen exchange; detection limit ... See more keywords
Stepwise Assembly of β-Sheet Structure during the Folding of an SH3 Domain Revealed by a Pulsed Hydrogen Exchange Mass Spectrometry Study.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemistry"
DOI: 10.1021/acs.biochem.7b00374
Abstract: Dissecting temporally the sequence of secondary structural changes, and determining how these specific changes modulate conformational heterogeneity, remain major goals of protein folding studies. In this study, the folding of the SH3 domain of PI3… read more here.
Keywords: sh3 domain; exchange mass; folding sh3; hydrogen exchange ... See more keywords
Computational Modeling of Molecular Structures Guided by Hydrogen-Exchange Data.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.1c00328
Abstract: Data produced by hydrogen-exchange monitoring experiments have been used in structural studies of molecules for several decades. Despite uncertainties about the structural determinants of hydrogen exchange itself, such data have successfully helped guide the structural… read more here.
Keywords: molecular structures; exchange; exchange data; hydrogen exchange ... See more keywords