A single point mutation converts GH84 O-GlcNAc hydrolases into phosphorylases. Experimental and theoretical evidence.
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DOI: 10.1021/jacs.9b09655
Abstract: Glycoside hydrolases and phosphorylases are two major classes of enzymes responsible for the cleavage of glycosidic bonds. Here we show that two GH84 O-GlcNAcase enzymes can be converted to efficient phosphorylases by a single point… read more here.
Keywords: mutation converts; point mutation; hydrolases phosphorylases; single point ... See more keywords