Mechanistic modeling based process analytical technology implementation for pooling in hydrophobic interaction chromatography
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Progress"
DOI: 10.1002/btpr.2758
Abstract: A major challenge in chromatography purification of therapeutic proteins is batch‐to‐batch variability with respect to impurity levels and product concentration in the feed. Mechanistic model can enable process analytical technology (PAT) implementation by predicting impact… read more here.
Keywords: chromatography; hydrophobic interaction; analytical technology; model ... See more keywords
Considerations for operational space definition and optimization of a no-salt flowthrough hydrophobic interaction chromatography purification step.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology progress"
DOI: 10.1002/btpr.3351
Abstract: No-salt flowthrough hydrophobic interaction chromatography (HIC) has been shown to effectively remove process and product-related impurities from bioprocess streams. In this publication, a panel of six antibodies has been used to demonstrate operating principles for… read more here.
Keywords: salt flowthrough; flowthrough hydrophobic; interaction chromatography; hydrophobic interaction ... See more keywords
Intermediate purification of CHO‐derived recombinant human Factor IX using hydrophobic interaction membrane‐based chromatography and its comparison to a sulfated resin
Sign Up to like & getrecommendations! Published in 2017 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201700226
Abstract: This work investigated the use of hydrophobic interaction membrane chromatography for intermediate purification of recombinant human Factor IX (rFIX) produced by CHO cells. The first purification step was based on a strong anion exchange monolith,… read more here.
Keywords: hydrophobic interaction; recombinant human; factor; intermediate purification ... See more keywords
Isolation of ellagic acid from pomegranate peel extract by hydrophobic interaction chromatography using graphene oxide grafted cotton fiber adsorbent.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of separation science"
DOI: 10.1002/jssc.201700896
Abstract: Ellagic acid, a natural polyphenol, was isolated from pomegranate peel extract by hydrophobic interaction using graphene oxide grafted cotton fiber as a stationary adsorbent. The grafted graphene oxide moieties served as hydrophobic interaction-binding sites for… read more here.
Keywords: graphene oxide; hydrophobic interaction; using graphene; ellagic acid ... See more keywords
Evaluation of hydrophobic‐interaction chromatography resins for purification of antibody‐drug conjugates using a mimetic model with adjustable hydrophobicity
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Separation Science"
DOI: 10.1002/jssc.201900895
Abstract: Abstract Antibody drug conjugates are cytotoxic pharmaceuticals, designed to destroy malignant cells. A cytotoxic molecule is attached to an antibody that binds specific to a cancer‐cell surface. Given the high toxicity of the drugs, strict… read more here.
Keywords: drug conjugates; hydrophobic interaction; antibody; antibody drug ... See more keywords
Retention Behavior of Polyethylene Glycol and Its Influence on Protein Elution on Hydrophobic Interaction Chromatography Media
Sign Up to like & getrecommendations! Published in 2018 at "Chromatographia"
DOI: 10.1007/s10337-018-3635-9
Abstract: The retention behavior of polyethylene glycol (PEG) on different types of hydrophobic interaction chromatography (HIC) resins containing butyl, octyl, and phenyl ligands was analyzed. An incomplete elution or splitting of the polymer peak into two… read more here.
Keywords: protein; behavior polyethylene; hydrophobic interaction; retention behavior ... See more keywords
Refolding with Simultaneous Purification of Recombinant Core Streptavidin Using Single-step High-performance Hydrophobic Interaction Chromatography
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-019-0041-6
Abstract: Streptavidin has applied to many areas including detection, purification, labeling, crosslinking and immobilization resulting in a high demand on its production. In this study, we report a method for preparation of recombinant core streptavidin (cSAV)… read more here.
Keywords: chromatography; hydrophobic interaction; recombinant core; streptavidin ... See more keywords
Characterization and quantification of succinimide using peptide mapping under low-pH conditions and hydrophobic interaction chromatography.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.11.021
Abstract: Characterization of asparagine deamidation and aspartic acid isomerization is an important aspect of biotherapeutic protein analysis due to the potential negative effect of these modifications on drug efficacy and stability. Succinimide has long been known… read more here.
Keywords: hydrophobic interaction; mapping low; interaction chromatography; peptide mapping ... See more keywords
Isolation and quantitation of exosomes isolated from human plasma via hydrophobic interaction chromatography using a polyester, capillary-channeled polymer fiber phase.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2019.07.035
Abstract: Exosomes are one class of extracellular vesicles (30-150 nm diameter) that are secreted by cells. These small vesicles hold a great deal of promise in disease diagnostics, as they display the same protein biomarkers as their… read more here.
Keywords: interaction chromatography; channeled polymer; isolation; hydrophobic interaction ... See more keywords
Rapid separation of blood plasma exosomes from low-density lipoproteins via a hydrophobic interaction chromatography method on a polyester capillary-channeled polymer fiber phase.
Sign Up to like & getrecommendations! Published in 2021 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2021.338578
Abstract: Exosomes are membrane-bound, cell-secreted vesicles, with sizes ranging from 30 to 150 nm. Exosomes in blood plasma have become proposed targets as measurable indicators of disease conditions. Current methods for plasma-based exosome isolation are time-consuming, complex,… read more here.
Keywords: hydrophobic interaction; plasma; low density; interaction chromatography ... See more keywords
Nanoparticle-doped polystyrene/polyacrylonitrile nanofiber membrane with hierarchical structure as promising protein hydrophobic interaction chromatography media
Sign Up to like & getrecommendations! Published in 2019 at "Composites Communications"
DOI: 10.1016/j.coco.2019.08.008
Abstract: Abstract Hydrophobic interaction chromatography (HIC) as an indispensable method for protein purification has attracted considerable attentions of researchers as well as biopharmaceutical industries. However, the operation pressure-drop and flow rate of the typical HIC porous… read more here.
Keywords: polystyrene polyacrylonitrile; interaction chromatography; hydrophobic interaction;