Stabilization and scale‐up of photosynthesis‐driven ω‐hydroxylation of nonanoic acid methyl ester by two‐liquid phase whole‐cell biocatalysis
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27006
Abstract: Photoautotrophic organisms are promising hosts for biocatalytic oxyfunctionalizations because they supply reduction equivalents as well as O2 via photosynthetic water oxidation. Thus far, research on photosynthesis‐driven bioprocesses mainly focuses on strain development and the proof… read more here.
Keywords: two liquid; phase; whole cell; liquid phase ... See more keywords
P450 BM3‐Catalyzed Regio‐ and Stereoselective Hydroxylation Aiming at the Synthesis of Phthalides and Isocoumarins
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DOI: 10.1002/cbic.201600685
Abstract: Cytochrome P450 BM3 monooxygenases are able to catalyze the regio‐ and stereoselective oxygenation of a broad range of substrates, with promising potential for synthetic applications. To study the suitability of P450 BM3 variants for stereoselective… read more here.
Keywords: p450 bm3; synthesis phthalides; hydroxylation; phthalides isocoumarins ... See more keywords
Methane Hydroxylation with Water as an Electron Donor under Light Irradiation in the Presence of Reconstituted Membranes Containing both Photosystem II and a Methane Monooxygenase
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DOI: 10.1002/cbic.201800324
Abstract: Methane/methanol conversion is one of the most important chemical reactions. Methane monooxygenases from methanotrophs are enzymes that catalyze methane/methanol conversion under mild conditions. Here we report the reconstitution of purified photosystem II (PSII) from Thermosynechococcus elongatus… read more here.
Keywords: methane; light irradiation; electron donor; hydroxylation ... See more keywords
Engineered Bacterial Flavin‐Dependent Monooxygenases for the Regiospecific Hydroxylation of Polycyclic Phenols
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202100480
Abstract: 4‐Hydroxyphenylacetate 3‐hydroxylase (4HPA3H), a flavin‐dependent monooxygenase from E. coli that catalyzes the hydroxylation of monophenols to catechols, was modified by rational redesign to convert also more bulky substrates, especially phenolic natural products like phenylpropanoids, flavones… read more here.
Keywords: flavin dependent; monooxygenases regiospecific; bacterial flavin; dependent monooxygenases ... See more keywords
Biochemical and Structural Insights into FIH‐Catalysed Hydroxylation of Transient Receptor Potential Ankyrin Repeat Domains
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200576
Abstract: Transient receptor potential (TRP) channels have important roles in environmental sensing in animals. Human TRP subfamily A member 1 (TRPA1) is responsible for sensing allyl isothiocyanate (AITC) and other electrophilic sensory irritants. TRP subfamily vanilloid… read more here.
Keywords: transient receptor; biochemical structural; receptor potential; hydroxylation ... See more keywords
Direct C(sp2)−H Hydroxylation of Arenes with Palladium(II)/Oxygen Using Sulfoximines as a Recyclable Directing Group
Sign Up to like & getrecommendations! Published in 2018 at "ChemCatChem"
DOI: 10.1002/cctc.201800064
Abstract: The aerobic palladium(II) catalysis presented herein offers facile entry to various substituted phenols through site‐selective C−H hydroxylation of arenes by using sulfoximines as a reusable directing group. The notable aspects of our method include the… read more here.
Keywords: oxygen; directing group; hydroxylation; palladium ... See more keywords
Mass Spectrometry and Bioinformatic Analysis of Hydroxylation-Dependent Protein-Protein Interactions.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7665-2_3
Abstract: Characterization of how a stimulus regulates the dynamics of protein-protein interaction is critical for understanding how a particular protein is regulated in an intracellular signaling network. Protein hydroxylation, which is a posttranslational modification catalyzed by… read more here.
Keywords: protein; hydroxylation dependent; mass spectrometry; hydroxylation ... See more keywords
Highly selective bile acid hydroxylation by the multifunctional bacterial P450 monooxygenase CYP107D1 (OleP)
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology Letters"
DOI: 10.1007/s10529-020-02813-4
Abstract: Objective Regio- and stereoselective hydroxylation of lithocholic acid (LCA) using CYP107D1 (OleP), a cytochrome P450 monooxygenase from the oleandomycin synthesis pathway of Streptomyces antibioticus . Results Co-expression of CYP107D1 from S. antibioticus and the reductase/ferredoxin… read more here.
Keywords: cyp107d1 olep; hydroxylation; monooxygenase cyp107d1; p450 monooxygenase ... See more keywords
Ni–Mg co-doped ZnO nanoparticles as a novel catalyst for hydroxylation of benzene to phenol
Sign Up to like & getrecommendations! Published in 2018 at "Research on Chemical Intermediates"
DOI: 10.1007/s11164-018-3333-0
Abstract: Mg and Ni were introduced as the novel co-doping materials for synthesizing Mg and Ni co-doping ZnO nanoparticles (NPs) through the simple wet chemical method. Photocatalyst properties of Mg and Ni co-doping ZnO NPs were… read more here.
Keywords: hydroxylation; benzene phenol; zno; hydroxylation benzene ... See more keywords
Screening of Fungi Isolates for C-4 Hydroxylation of R-2-Phenoxypropionic Acid Based on a Novel 96-Well Microplate Assay Method
Sign Up to like & getrecommendations! Published in 2020 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-020-03303-z
Abstract: R-2-(4-Hydroxyphenoxy)propionic acid (R-HPPA) is a pivotal intermediate for the synthesis of aryloxyphenoxypropionate (APP) herbicide. To rapidly screen microbial isolates with the capacity of hydroxylating R-2-phenoxypropionic acid to R-HPPA from various environmental samples, a convenient and… read more here.
Keywords: assay method; hydroxylation; well microplate; microplate assay ... See more keywords
Metal-free hydroxylation of tertiary ketones under intensified and scalable continuous flow conditions
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DOI: 10.1007/s41981-019-00073-6
Abstract: An intensified and scalable continuous flow process is presented for the hydroxylation of enolizable tertiary ketones. The procedure relies on molecular oxygen, metal-free conditions and a low toxicity solvent (DMSO). The reaction is optimized on… read more here.
Keywords: intensified scalable; tertiary ketones; scalable continuous; hydroxylation ... See more keywords