High-Resolution Analysis of 5-Hydroxymethylcytosine by TET-Assisted Bisulfite Sequencing.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0876-0_25
Abstract: DNA cytosine modification is an important epigenetic mechanism that serves critical functions in a variety of biological processes in development and disease. 5-Methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are the two most common epigenetic marks found… read more here.
Keywords: hydroxymethylcytosine; bisulfite sequencing; tet assisted; tet ... See more keywords
Modular Oxidation of Cytosine Modifications and Their Application in Direct and Quantitative Sequencing of 5-Hydroxymethylcytosine
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DOI: 10.1021/jacs.3c01663
Abstract: Selective, efficient, and controllable oxidation of cytosine modifications is valuable for epigenetic analyses, yet only limited progress has been made. Here, we present two modular chemical oxidation reactions: conversion of 5-hydroxymethylcytosine (5hmC) into 5-formylcytosine (5fC)… read more here.
Keywords: oxidation; direct quantitative; oxidation cytosine; cytosine modifications ... See more keywords
Epigenomic landscape of 5-hydroxymethylcytosine reveals its transcriptional regulation of lncRNAs in colorectal cancer
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DOI: 10.1038/bjc.2016.457
Abstract: Background:DNA methylation at the 5 position of cytosine (5mC) can be converted to 5-hydroxymethylcytosine (5hmC) by the ten–eleven translocation family. The loss of global levels of 5hmC has been regarded as a hallmark in various… read more here.
Keywords: hydroxymethylcytosine; epigenomic landscape; colorectal cancer; cancer ... See more keywords
5-hydroxymethylcytosine in DNA repair: A new player or a red herring?
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DOI: 10.1080/15384101.2017.1346761
Abstract: ABSTRACT Active DNA demethylation performed by ten-eleven translocation (TET) enzymes produces 5-hydroxymethylcytosines, 5-formylcytosines, and 5-carboxylcytosines. Recent observations suggest that 5-hydroxymethylcytosine is a stable epigenetic mark rather than merely an intermediate of DNA demethylation. However, the… read more here.
Keywords: hydroxymethylcytosine; dna repair; dna; player ... See more keywords
Global DNA 5-hydroxymethylcytosine level and its chromosomal distribution in four rye (Secale L.) species.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of experimental botany"
DOI: 10.1093/jxb/erad102
Abstract: The rye genome has a large size with a high level of cytosine methylation, which makes it particularly convenient for studying the occurrence of potential cytosine demethylation intermediates. Both the global level of 5-hydroxymethylcytosine (5hmC)… read more here.
Keywords: four rye; secale; dna; level ... See more keywords
Prediction of RNA 5-Hydroxymethylcytosine Modifications Using Deep Learning
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DOI: 10.1109/access.2021.3049146
Abstract: It is becoming increasingly clear that RNA 5-hydroxymethylcytosine (5hmC), which plays an important role in several biological processes, is one of the most important objects of study in the field of RNA epigenetics. Biochemical experiments… read more here.
Keywords: rna hydroxymethylcytosine; hydroxymethylcytosine modifications; hydroxymethylcytosine; model ... See more keywords
Basal cell carcinoma colonized by lentigo maligna melanoma, with perineural invasion by both neoplasms
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DOI: 10.1111/cup.13886
Abstract: REFERENCES 1. Wiesner T, Kutzner H, Cerroni L, Mihm MC Jr, Busam KJ, Murali R. Genomic aberrations in spitzoid melanocytic tumours and their implications for diagnosis, prognosis and therapy. Pathology. 2016;48(2): 113-131. 2. Lian CG,… read more here.
Keywords: melanoma; hydroxymethylcytosine; cutan pathol; pathology ... See more keywords
Hyperglycemia affects global 5-methylcytosine and 5-hydroxymethylcytosine in blood genomic DNA through upregulation of SIRT6 and TETs
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DOI: 10.1186/s13148-019-0660-y
Abstract: BackgroundAccumulating evidence suggests that epigenetic changes play key roles in the pathogenesis of type 2 diabetes mellitus (T2DM). However, the dynamic regulation of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in diabetic peripheral blood DNA remains to… read more here.
Keywords: hydroxymethylcytosine; genomic dna; sirt6 tets; methylcytosine ... See more keywords
Integrative Analysis of 5-Hydroxymethylcytosine and Transcriptional Profiling Identified 5hmC-Modified lncRNA Panel as Non-Invasive Biomarkers for Diagnosis and Prognosis of Pancreatic Cancer
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DOI: 10.3389/fcell.2022.845641
Abstract: Pancreatic adenocarcinoma (PAAD) is the fourth leading cause of cancer-related deaths worldwide. 5-Hydroxymethylcytosine (5hmC)-mediated epigenetic regulation has been reported to be involved in cancer pathobiology and has emerged to be promising biomarkers for cancer diagnosis… read more here.
Keywords: hydroxymethylcytosine; cancer; 5hmc modified; diagnosis prognosis ... See more keywords
PRDM9 activity depends on HELLS and promotes local 5-hydroxymethylcytosine enrichment
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DOI: 10.7554/elife.57117
Abstract: Meiotic recombination starts with the formation of DNA double-strand breaks (DSBs) at specific genomic locations that correspond to PRDM9-binding sites. The molecular steps occurring from PRDM9 binding to DSB formation are unknown. Using proteomic approaches… read more here.
Keywords: hydroxymethylcytosine; enrichment; prdm9 binding; dsb activity ... See more keywords