Protein engineering of antifreeze proteins reveals that their activity scales with the area of the ice‐binding site
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Letters"
DOI: 10.1002/1873-3468.14552
Abstract: Antifreeze proteins (AFPs) protect organisms from freezing by binding to ice crystals to prevent their growth. Here, we have investigated how the area of an AFP's ice‐binding site (IBS) changes its antifreeze activity. The polyproline… read more here.
Keywords: antifreeze; area; activity; antifreeze proteins ... See more keywords
Isolation and Characterization of Ice-Binding Proteins from Higher Plants.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0660-5_21
Abstract: The characterization of ice-binding proteins (IBPs) from plants can involve many techniques, a few of which are presented here. Chief among these methods are tests for ice recrystallization inhibition, an activity characteristic of plant IBPs.… read more here.
Keywords: binding proteins; activity; ice binding; characterization ice ... See more keywords
Ice-binding proteins-not only for ice growth control.
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.3170
Abstract: Ice-binding proteins (IBPs) evolved in organisms living in cold ecosystems as a protective measure against freezing stresses. Some IBPs prevent their hosts from freezing mainly by depression of the freezing point of the body fluids… read more here.
Keywords: growth control; binding proteins; ice binding; ice growth ... See more keywords
Properties of phase transition of ice binding protein from Arctic yeast (LeIBP) utilizing differential scanning calorimetry (DSC) and Raman spectroscopy.
Sign Up to like & getrecommendations! Published in 2018 at "Cryobiology"
DOI: 10.1016/j.cryobiol.2018.10.005
Abstract: Ice binding proteins (IBPs) have been attracting significant interest on account of their characteristic of inhibiting ice growth and recrystallization. Owing to their unique characteristics, IBPs have been studied for applications in food, pharmaceuticals, and… read more here.
Keywords: dsc raman; ice binding; spectroscopy; raman spectroscopy ... See more keywords
Importance of rigidity of ice-binding protein (FfIBP) for hyperthermal hysteresis activity and microbial survival.
Sign Up to like & getrecommendations! Published in 2022 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2022.02.032
Abstract: Ice-binding proteins (IBPs) are well-characterized proteins responsible for the cold-adaptation mechanisms. Despite extensive structural and biological investigation of IBPs and antifreeze proteins, only a few studies have considered the relationship between protein stabilization and thermal… read more here.
Keywords: ffibp; hysteresis activity; ice binding; activity ... See more keywords
Cryo-protective effect of ice-binding peptides derived from collagen hydrolysates on the frozen dough and its ice-binding mechanisms
Sign Up to like & getrecommendations! Published in 2020 at "Lwt - Food Science and Technology"
DOI: 10.1016/j.lwt.2020.109678
Abstract: Abstract Ice-bonding collagen peptides (IBCPs) from bovine bone collagen hydrolysates were isolated by an improved ice-affinity adsorption system. Then its breadmaking performance in the frozen dough and ice-binding mechanism were investigated. The results showed that… read more here.
Keywords: ice binding; dough ice; ice; frozen dough ... See more keywords
The protective effect of Leucosporidium-derived ice-binding protein (LeIBP) on bovine oocytes and embryos during vitrification.
Sign Up to like & getrecommendations! Published in 2020 at "Theriogenology"
DOI: 10.1016/j.theriogenology.2020.04.016
Abstract: Ice-binding proteins (IBPs) facilitate organism survival under extreme conditions by inhibiting thermal hysteresis and ice recrystallization. IBPs have been widely used as cryoprotectants to cryopreserve mammalian gametes and embryos. In the present study, we evaluated… read more here.
Keywords: bovine; oocytes embryos; ice binding; derived ice ... See more keywords
Whole-genome sequencing of the endemic Antarctic fungus Antarctomyces pellizariae reveals an ice-binding protein, a scarce set of secondary metabolites gene clusters and provides insights on Thelebolales phylogeny.
Sign Up to like & getrecommendations! Published in 2020 at "Genomics"
DOI: 10.1016/j.ygeno.2020.05.004
Abstract: The snow-covered surfaces of Antarctica comprise an extreme environment that favors the development of life forms with adaptations to adverse low-temperature habitats. The ability to survive and such temperatures might involve the production of antifreeze… read more here.
Keywords: endemic antarctic; ice binding; antarctic fungus; binding protein ... See more keywords
Inhibition of Ice Recrystallization by Nanotube-Forming Cyclic Peptides
Sign Up to like & getrecommendations! Published in 2022 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.1c01267
Abstract: While most native ice-binding proteins are rigid, artificial (macro)molecular ice-binders are usually flexible. Realizing a regular array with precisely positioned ice-binding motifs on synthetic proteins, (macro)molecular ice-binders are thus challenging. Here, we exploit the predictable… read more here.
Keywords: cyclic peptides; ice; spectroscopy; ice recrystallization ... See more keywords
Bioinspired Ice-Binding Materials for Tissue and Organ Cryopreservation.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.2c00203
Abstract: Cryopreservation of tissues and organs can bring transformative changes to medicine and medical science. In the past decades, limited progress has been achieved, although cryopreservation of tissues and organs has long been intensively pursued. One… read more here.
Keywords: ice; tissues organs; cryopreservation; binding materials ... See more keywords
An ice-binding protein from an Arctic grass, Leymus mollis.
Sign Up to like & getrecommendations! Published in 2020 at "F1000Research"
DOI: 10.12688/f1000research.24328.1
Abstract: Several cold-hardy grasses have been shown to have ice-binding proteins (IBPs) that protect against freeze-thaw injury. Here, we looked for IBP activity in an Alaskan coastal grass that had not previously been examined, Leymus mollis… read more here.
Keywords: grass; leymus mollis; iri domain; ice binding ... See more keywords