Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase
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DOI: 10.1038/s41598-019-46357-8
Abstract: We developed a novel and convenient method for rapidly identifying CRISPR/Cas9-based genome-edited biallelic knockout (KO) cells/individuals carrying insertions or deletions of a few nucleotides (indels) by performing PCR on genomic DNA samples under stringent conditions… read more here.
Keywords: crispr cas9; novel convenient; identifying crispr; biallelic mutants ... See more keywords