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Published in 2021 at "Nature Protocols"
DOI: 10.1038/s41596-020-00453-6
Abstract: Digested genome sequencing (Digenome-seq) is a highly sensitive, easy-to-carry-out, cell-free method for experimentally identifying genome-wide off-target sites of programmable nucleases and deaminases (also known as base editors). Genomic DNA is digested in vitro using clustered…
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Keywords:
genome wide;
dna;
identifying genome;
target sites ... See more keywords