Soluble expression of IGF1 fused to DsbA in SHuffle™ T7 strain: optimization of expression and purification by Box-Behnken design
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DOI: 10.1007/s00253-019-09719-w
Abstract: Production of insulin-like growth factor 1 (IGF1) in Escherichia coli mostly results in the formation of inclusion bodies. In the present study, IGF1 was fused to disulfide bond oxidoreductase A (DsbA) and expressed in SHuffle™… read more here.
Keywords: dsba; igf1; expression; optimization ... See more keywords