Type III CRISPR-Cas systems: when DNA cleavage just isn't enough.
Sign Up to like & getrecommendations! Published in 2017 at "Current opinion in microbiology"
DOI: 10.1016/j.mib.2017.08.003
Abstract: Type III CRISPR-Cas systems have a unique targeting mechanism that requires the transcription of the DNA target and results in the degradation of not only the genome of the invader but also its transcripts. Here… read more here.
Keywords: crispr cas; iii crispr; type iii; cas systems ... See more keywords
If You'd Like to Stop a Type III CRISPR Ribonuclease, Then You Should Put a Ring (Nuclease) on It.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular cell"
DOI: 10.1016/j.molcel.2018.10.048
Abstract: Athukoralage et al. (2018) identify a new class of nuclease that degrades cyclic oligoadenylate (cOA), a second messenger that activates non-specific RNA degradation by the type III CRISPR-Cas accessory RNase Csm6/Csx1. This discovery provides a mechanism… read more here.
Keywords: nuclease; iii crispr; type iii;
New Type III CRISPR variant and programmable RNA targeting tool: Oh, thank heaven for Cas7-11.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular cell"
DOI: 10.1016/j.molcel.2021.10.014
Abstract: Özcan et al. (2021) and van Beljouw et al. (2021) characterize a novel Type III-E CRISPR-Cas subtype, composed of a single polypeptide with crRNA processing and sequence-specific RNA cleavage activities, that provides a new RNA knockdown tool… read more here.
Keywords: iii crispr; type iii; new type;
Characterization of a novel type III CRISPR-Cas effector provides new insights into the allosteric activation and suppression of the Cas10 DNase
Sign Up to like & getrecommendations! Published in 2020 at "Cell Discovery"
DOI: 10.1038/s41421-020-0160-4
Abstract: Antiviral defense by type III CRISPR-Cas systems relies on two distinct activities of their effectors: the RNA-activated DNA cleavage and synthesis of cyclic oligoadenylate. Both activities are featured as indiscriminate nucleic acid cleavage and subjected… read more here.
Keywords: iii; crispr cas; target; activation ... See more keywords
Structure of Csx1-cOA4 complex reveals the basis of RNA decay in Type III-B CRISPR-Cas
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-12244-z
Abstract: Type III CRISPR-Cas multisubunit complexes cleave ssRNA and ssDNA. These activities promote the generation of cyclic oligoadenylate (cOA), which activates associated CRISPR-Cas RNases from the Csm/Csx families, triggering a massive RNA decay to provide immunity… read more here.
Keywords: crispr cas; cas; coa4; iii crispr ... See more keywords
Type III-A CRISPR immunity promotes mutagenesis of staphylococci.
Sign Up to like & getrecommendations! Published in 2021 at "Nature"
DOI: 10.1038/s41586-021-03440-3
Abstract: Horizontal gene transfer and mutation are the two major drivers of microbial evolution that enable bacteria to adapt to fluctuating environmental stressors1. Clustered, regularly interspaced, short palindromic repeats (CRISPR) systems use RNA-guided nucleases to direct… read more here.
Keywords: iii crispr; crispr systems; type iii;
Tolerance of Sulfolobus SMV1 virus to the immunity of I-A and III-B CRISPR-Cas systems in Sulfolobus islandicus
Sign Up to like & getrecommendations! Published in 2019 at "RNA Biology"
DOI: 10.1080/15476286.2018.1460993
Abstract: ABSTRACT Sulfolobus islandicus Rey15A encodes one Type I-A and two Type III-B systems, all of which are active in mediating nucleic acids interference. However, the effectiveness of each CRISPR system against virus infection was not… read more here.
Keywords: immunity; crispr; sulfolobus islandicus; crispr immunity ... See more keywords
Type III-A CRISPR-associated protein Csm6 degrades cyclic hexa-adenylate activator using both CARF and HEPN domains
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa634
Abstract: Abstract The type III CRISPR–Cas systems provide immunity against invading nucleic acids through the coordinated transcription-dependent DNA targeting and cyclic adenylate (cAn)-activated RNA degradation. Here, we show that both these pathways contribute to the Streptococcus… read more here.
Keywords: hepn domains; hepn; carf hepn; iii crispr ... See more keywords
The CRISPR ancillary effector Can2 is a dual-specificity nuclease potentiating type III CRISPR defence
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab073
Abstract: Abstract Cells and organisms have a wide range of mechanisms to defend against infection by viruses and other mobile genetic elements (MGE). Type III CRISPR systems detect foreign RNA and typically generate cyclic oligoadenylate (cOA)… read more here.
Keywords: crispr; iii crispr; can2; effector ... See more keywords
Structural basis of cyclic oligoadenylate degradation by ancillary Type III CRISPR-Cas ring nucleases
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab1130
Abstract: Abstract Type III CRISPR-Cas effector systems detect foreign RNA triggering DNA and RNA cleavage and synthesizing cyclic oligoadenylate molecules (cA) in their Cas10 subunit. cAs act as a second messenger activating auxiliary nucleases, leading to… read more here.
Keywords: iii crispr; type iii; crispr cas; type ... See more keywords
Molecular basis of stepwise cyclic tetra-adenylate cleavage by the type III CRISPR ring nuclease Crn1/Sso2081
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1101/2022.10.01.510428
Abstract: The cyclic oligoadenylates (cOAs) act as second messengers of type III CRISPR immunity system through activating the auxiliary nucleases for indiscriminate RNA degradation. The cOA-degrading nucleases (ring nucleases) provide an ‘off-switch’ regulation of the signaling,… read more here.
Keywords: ring nuclease; cleavage; type iii; nuclease crn1 ... See more keywords