Purification of Recombinant ESCRT-III Proteins and Their Use in Atomic Force Microscopy and In Vitro Binding and Phosphorylation Assays.
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DOI: 10.1007/978-1-4939-9492-2_15
Abstract: The endosomal sorting complex required for transport (ESCRT)-III proteins are known to assemble into filaments that mediate membrane remodeling and fission in various biological processes, including the formation of endosomal multivesicular bodies, viral budding, cytokinesis,… read more here.
Keywords: escrt iii; purification; iii proteins; microscopy ... See more keywords
LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells
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DOI: 10.1073/pnas.1613916114
Abstract: Significance The molecular mechanism for sealing newly formed nuclear envelopes was unclear until the recent discovery that endosomal sorting complexes required for transport III (ESCRT-III) proteins mediate this process. Cmp7p (CHMP7), in particular, was identified… read more here.
Keywords: nuclear envelope; iii proteins; escrt iii;