Evaluation of microsatellite instability testing through cell-free DNA sequencing
Sign Up to like & getrecommendations! Published in 2019 at "Annals of Oncology"
DOI: 10.1093/annonc/mdz239.026
Abstract: Abstract Background Microsatellite instability (MSI) status has been approved by FDA to select for patients with metastatic tumors for cancer immunotherapy treatments. Additionally, MSI status is used in assessment of prognosis and treatment choices in… read more here.
Keywords: time employment; full part; time; illumina ... See more keywords
Measurement error and variant-calling in deep Illumina sequencing of HIV
Sign Up to like & getrecommendations! Published in 2019 at "Bioinformatics"
DOI: 10.1093/bioinformatics/bty919
Abstract: MOTIVATION Next-generation deep sequencing of viral genomes, particularly on the Illumina platform, is increasingly applied in HIV research. Yet, there is no standard protocol or method used by the research community to account for measurement… read more here.
Keywords: variant calling; erroneous variants; calling pipelines; error ... See more keywords
Draft genome assemblies using sequencing reads from Oxford Nanopore Technology and Illumina platforms for four species of North American Fundulus killifish
Sign Up to like & getrecommendations! Published in 2020 at "GigaScience"
DOI: 10.1093/gigascience/giaa067
Abstract: Abstract Background Whole-genome sequencing data from wild-caught individuals of closely related North American killifish species (Fundulus xenicus, Fundulus catenatus, Fundulus nottii, and Fundulus olivaceus) were obtained using long-read Oxford Nanopore Technology (ONT) PromethION and short-read… read more here.
Keywords: nanopore technology; draft; illumina; north american ... See more keywords
Comparative analysis of 7 short-read sequencing platforms using the Korean Reference Genome: MGI and Illumina sequencing benchmark for whole-genome sequencing
Sign Up to like & getrecommendations! Published in 2021 at "GigaScience"
DOI: 10.1093/gigascience/giab014
Abstract: Abstract Background DNBSEQ-T7 is a new whole-genome sequencer developed by Complete Genomics and MGI using DNA nanoball and combinatorial probe anchor synthesis technologies to generate short reads at a very large scale—up to 60 human… read more here.
Keywords: short read; whole genome; illumina; rate ... See more keywords
Simultaneous SARS-CoV-2 Genome Sequencing of 384 Samples on an Illumina MiSeq Instrument through Protocol Optimization
Sign Up to like & getrecommendations! Published in 2022 at "Genes"
DOI: 10.3390/genes13091648
Abstract: In the present study, we propose a high-throughput sequencing protocol using aNextera XT Library DNA kit on an Illumina MiSeq instrument. We made major modifications to this library preparation in order to multiplex 384 samples… read more here.
Keywords: illumina; miseq instrument; 384 samples; illumina protocol ... See more keywords
Highly comparable metabarcoding results from MGI-Tech and Illumina sequencing platforms
Sign Up to like & getrecommendations! Published in 2021 at "PeerJ"
DOI: 10.7717/peerj.12254
Abstract: With the developments in DNA nanoball sequencing technologies and the emergence of new platforms, there is an increasing interest in their performance in comparison with the widely used sequencing-by-synthesis methods. Here, we test the consistency… read more here.
Keywords: metabarcoding results; mgi tech; illumina; sequencing platforms ... See more keywords
Random sampling causes the low reproducibility of rare eukaryotic OTUs in Illumina COI metabarcoding
Sign Up to like & getrecommendations! Published in 2017 at "PeerJ"
DOI: 10.7717/peerj.3006
Abstract: DNA metabarcoding, the PCR-based profiling of natural communities, is becoming the method of choice for biodiversity monitoring because it circumvents some of the limitations inherent to traditional ecological surveys. However, potential sources of bias that… read more here.
Keywords: sample; rare otus; otus; reproducibility ... See more keywords