Rapid Detection of γ-H2Av Foci in Ex Vivo MMS-Treated Drosophila Imaginal Discs.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7187-9_19
Abstract: In Drosophila melanogaster, DNA double-strand breaks (DSBs) created by exposure to gamma or X-ray radiation can be quantified by immunofluorescent detection of phosphorylated histone H2Av (γ-H2Av) foci in imaginal disc tissues. This technique has been… read more here.
Keywords: imaginal discs; detection; h2av foci; mms ... See more keywords
Angiotensin-converting enzyme Ance is cooperatively regulated by Mad and Pannier in Drosophila imaginal discs
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DOI: 10.1038/s41598-017-13487-w
Abstract: Angiotensin-converting enzyme (ACE) is an evolutionarily conserved peptidyl dipeptidase. Mammalian ACE converts angiotensin I to the active vasoconstrictor angiotensin II, thus playing a critical role for homeostasis of the renin-angiotensin system. In Drosophila, the ACE… read more here.
Keywords: imaginal discs; ance expression; expression; angiotensin ... See more keywords
Fly-QMA: Automated analysis of mosaic imaginal discs in Drosophila
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DOI: 10.1371/journal.pcbi.1007406
Abstract: Mosaic analysis provides a means to probe developmental processes in situ by generating loss-of-function mutants within otherwise wildtype tissues. Combining these techniques with quantitative microscopy enables researchers to rigorously compare RNA or protein expression across… read more here.
Keywords: microscopy; analysis; fly qma; mosaic ... See more keywords
Mass Purification Protocol for Drosophila melanogaster Wing Imaginal Discs: An Alternative to Dissection to Obtain Large Numbers of Disc Cells
Sign Up to like & getrecommendations! Published in 2022 at "Biology"
DOI: 10.3390/biology11101384
Abstract: Simple Summary Drosophila melanogaster, also known as the fruit fly, is a widely used organism model, especially for genetic studies or as a model for pathologies. The Drosophila genome is well known and conserved within… read more here.
Keywords: imaginal discs; wing imaginal; dissection; protocol ... See more keywords