Simultaneous Analysis of Phenotype and Cytogenetics Using Imaging Flow Cytometry: Time to Teach Old Dogs New Tricks
Sign Up to like & getrecommendations! Published in 2019 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23776
Abstract: THE importance of cytogenetic abnormalities in clinical diagnosis and response has been well established in particular for hematological malignancies with the diagnostic tools being microscopy-based karyotyping or fluorescence in situ hybridization (FISH). For chronic lymphocytic… read more here.
Keywords: spot; imaging flow; hybridization; intensity ... See more keywords
Deep learning‐enabled imaging flow cytometry for high‐speed Cryptosporidium and Giardia detection
Sign Up to like & getrecommendations! Published in 2021 at "Cytometry Part A"
DOI: 10.1002/cyto.a.24321
Abstract: Imaging flow cytometry has become a popular technology for bioparticle image analysis because of its capability of capturing thousands of images per second. Nevertheless, the vast number of images generated by imaging flow cytometry imposes… read more here.
Keywords: cryptosporidium giardia; deep learning; imaging flow; flow cytometry ... See more keywords
The potential of multispectral imaging flow cytometry for environmental monitoring
Sign Up to like & getrecommendations! Published in 2022 at "Cytometry Part A"
DOI: 10.1002/cyto.a.24658
Abstract: Environmental monitoring involves the quantification of microscopic cells and particles such as algae, plant cells, pollen, or fungal spores. Traditional methods using conventional microscopy require expert knowledge, are time‐intensive and not well‐suited for automated high… read more here.
Keywords: multispectral imaging; flow cytometry; imaging flow; monitoring ... See more keywords
Optical time-stretch imaging flow cytometry in the compressed domain.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of biophotonics"
DOI: 10.1002/jbio.202300096
Abstract: Imaging flow cytometry based on optical time-stretch (OTS) imaging combined with a microfluidic chip attracts much attention in the large-scale single-cell analysis due to its high-throughput, high-precision and label-free operation. Compressive sensing has been integrated… read more here.
Keywords: time stretch; flow cytometry; optical time; imaging flow ... See more keywords
Detection of methicillin‐resistant Staphylococcus aureus persistence in osteoblasts using imaging flow cytometry
Sign Up to like & getrecommendations! Published in 2020 at "MicrobiologyOpen"
DOI: 10.1002/mbo3.1017
Abstract: Methicillin‐resistant S. aureus has been reported as the main pathogen involved in chronic infections, osteomyelitis, and prosthetic joint infections. The host/pathogen interaction is dynamic and requires several changes to promote bacterial survival. Here, we focused… read more here.
Keywords: staphylococcus aureus; cell culture; imaging flow; methicillin resistant ... See more keywords
Using Imaging Flow Cytometry to Quantify Neutrophil Phagocytosis.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0154-9_10
Abstract: Neutrophils are professional phagocytes that are important for innate host defenses against pathogens and resolution of inflammation. Traditionally, the phagocytic capacity of neutrophils was quantified by enumeration of cells containing either internalized or bound bacteria… read more here.
Keywords: using imaging; imaging flow; flow cytometry; quantify neutrophil ... See more keywords
Analysis of the Interaction of Human Neuroblastoma Cell-Derived Cytochalasin B Induced Membrane Vesicles with Mesenchymal Stem Cells Using Imaging Flow Cytometry
Sign Up to like & getrecommendations! Published in 2022 at "Bionanoscience"
DOI: 10.1007/s12668-021-00931-5
Abstract: At present, there is an increasing interest in the potential role of extracellular vesicles (EVs), acting as multi-signal messengers of the tumor stroma, in the development and progression of tumor. Tumor cell-derived EVs are considered… read more here.
Keywords: tumor; mscs; using imaging; cell derived ... See more keywords
Multiplexed quantification of autophagic flux by imaging flow cytometry.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2021.02.005
Abstract: Imaging flow cytometry allows for the quantitative assessment of fluorescent signals at the subcellular level. Here, we describe the use of a biosensor cell line, namely, U2OS osteosarcoma cells equipped with a fusion protein comprising… read more here.
Keywords: flux imaging; autophagic flux; fluorescent; imaging flow ... See more keywords
Imaging Flow Cytometry Illuminates New Dimensions of Amyloid Peptide-Membrane Interactions.
Sign Up to like & getrecommendations! Published in 2020 at "Biophysical journal"
DOI: 10.1016/j.bpj.2020.01.018
Abstract: Membrane interactions of amyloidogenic proteins constitute central determinants both in protein aggregation as well as in amyloid cytotoxicity. Most reported studies of amyloid peptide-membrane interactions have employed model membrane systems combined with application of spectroscopy… read more here.
Keywords: amyloid peptide; imaging flow; 106 126; flow cytometry ... See more keywords
High-throughput microfluidic imaging flow cytometry.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2018.08.002
Abstract: Recently, microfluidic-based flow cytometry platforms have been shown to be powerful tools for the manipulation and analysis of single cells and micron-sized particles in flow. That said, current microfluidic flow cytometers are limited in both… read more here.
Keywords: cytometry; imaging flow; flow cytometry; throughput microfluidic ... See more keywords
Imaging flow cytometry and GST pulldown assays provide new insights into channel catfish leukocyte immune‐type receptor‐mediated phagocytic pathways
Sign Up to like & getrecommendations! Published in 2017 at "Developmental and Comparative Immunology"
DOI: 10.1016/j.dci.2016.10.011
Abstract: ABSTRACT Channel catfish (Ictalurus punctatus) leukocyte immune‐type receptors (IpLITRs) control various innate immune cell effector responses including the phagocytic process. This large immunoregulatory receptor family also consists of multiple receptor‐types with variable signaling abilities that… read more here.
Keywords: immune; phagocytosis; imaging flow; flow cytometry ... See more keywords