High-Throughput Calcium Imaging Screen of Toxins' Function in Dissociated Sensory Neurons.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9845-6_15
Abstract: Many toxins from a variety of venomous animals and plants have evolved to target neuronal ion channels and receptors. However, a significant obstacle in the study of these toxins is the finding and characterization of… read more here.
Keywords: screen toxins; calcium imaging; throughput calcium; calcium ... See more keywords
A multi-phenotypic imaging screen to identify bacterial effectors by exogenous expression in a HeLa cell line
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DOI: 10.1038/sdata.2018.81
Abstract: We present a high-content screen (HCS) for the simultaneous analysis of multiple phenotypes in HeLa cells expressing an autophagy reporter (mcherry-LC3) and one of 224 GFP-fused proteins from the Crohn’s Disease (CD)-associated bacterium, Adherent Invasive… read more here.
Keywords: identify bacterial; cell; multi phenotypic; imaging screen ... See more keywords
Genome-wide imaging screen uncovers molecular determinants of arsenite-induced protein aggregation and toxicity.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of cell science"
DOI: 10.1242/jcs.258338
Abstract: The toxic metalloid arsenic causes widespread misfolding and aggregation of cellular proteins. How these protein aggregates are formed in vivo, the mechanisms by which they affect cells, and how cells prevent their accumulation is not… read more here.
Keywords: genome wide; aggregation; wide imaging; toxicity ... See more keywords