Quantitative Super-Resolution Imaging of Small RNAs in Bacterial Cells.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7634-8_12
Abstract: We present a method for the quantification of small regulatory RNAs (sRNAs) in bacteria, by combining single-molecule fluorescence in situ hybridization (smFISH), super-resolved single-fluorophore microscopy, and clustering analysis. Compared to smFISH imaging with diffraction-limited fluorescence… read more here.
Keywords: bacterial cells; resolution imaging; imaging small; microscopy ... See more keywords
Live-cell imaging of small nucleolar RNA tagged with the broccoli aptamer in yeast.
Sign Up to like & getrecommendations! Published in 2018 at "FEMS yeast research"
DOI: 10.1093/femsyr/foy093
Abstract: The development of the RNA 'vegetable' aptamers, Spinach and Broccoli, has simplified RNA imaging, especially in live cells. These RNA aptamers interact with a fluorophore (DFHBI or DFHBI-1T) to produce a green fluorescence signal. Although… read more here.
Keywords: cell imaging; live cell; imaging small; broccoli aptamer ... See more keywords
The imaging of small pulmonary nodules.
Sign Up to like & getrecommendations! Published in 2017 at "Translational lung cancer research"
DOI: 10.21037/tlcr.2017.02.02
Abstract: Lung cancer is the leading cause of cancer death worldwide. The major goal in lung cancer research is the improvement of long-term survival. Pulmonary nodules have high clinical importance, they may not only prove to… read more here.
Keywords: imaging small; pulmonary nodules; lung cancer; small pulmonary ... See more keywords