Immortalization of human primary prostate epithelial cells via CRISPR inactivation of the CDKN2A locus and expression of telomerase
Sign Up to like & getrecommendations! Published in 2020 at "Prostate Cancer and Prostatic Diseases"
DOI: 10.1038/s41391-020-00274-4
Abstract: Background Immortalization of primary prostate epithelial cells (PrEC) with just hTERT expression is particularly inefficient in the absence of DNA tumor viral proteins or p16 INK4A knockdown. Materials and methods Here, we describe the establishment… read more here.
Keywords: prostate; primary prostate; cell; expression ... See more keywords
Abstract 5818: The immortalization process as a therapeutic target
Sign Up to like & getrecommendations! Published in 2020 at "Cancer Research"
DOI: 10.1158/1538-7445.am2020-5818
Abstract: Normal human somatic cells have a finite lifespan and intact tumor suppressor barriers, whereas most carcinoma cells have gained immortality and overcome multiple tumor suppressor barriers. We have developed a comprehensive human mammary epithelial cell… read more here.
Keywords: immortalization process; process therapeutic; cancer; immortalization ... See more keywords
Novel cell lines derived from Chinese hamster kidney tissue
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0266061
Abstract: Immortalized kidney cell lines are widely used in basic and applied research such as cell permeability tests and drug screening. Although many cell lines have been established from kidney tissues, the immortalization process has not… read more here.
Keywords: cell; immortalization; kidney; chk cells ... See more keywords
Species-specific functions of Epstein-Barr virus nuclear antigen 2 (EBNA2) reveal dual roles for initiation and maintenance of B cell immortalization
Sign Up to like & getrecommendations! Published in 2017 at "PLoS Pathogens"
DOI: 10.1371/journal.ppat.1006772
Abstract: Epstein-Barr virus (EBV) and related lymphocryptoviruses (LCV) from non-human primates infect B cells, transform their growth to facilitate life-long viral persistence in the host, and contribute to B cell oncogenesis. Co-evolution of LCV with their… read more here.
Keywords: epstein barr; immortalization; ebna2; barr virus ... See more keywords
Integration of ALV into CTDSPL and CTDSPL2 genes in B-cell lymphomas promotes cell immortalization, migration and survival.
Sign Up to like & getrecommendations! Published in 2017 at "Oncotarget"
DOI: 10.18632/oncotarget.19328
Abstract: Avian leukosis virus induces tumors in chickens by integrating into the genome and altering expression of nearby genes. Thus, ALV can be used as an insertional mutagenesis tool to identify novel genes involved in tumorigenesis.… read more here.
Keywords: integration; ctdspl ctdspl2; cell lymphomas; migration ... See more keywords
Lactate Dehydrogenase B Is Required for Pancreatic Cancer Cell Immortalization Through Activation of Telomerase Activity
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Oncology"
DOI: 10.3389/fonc.2022.821620
Abstract: Telomerase activity is elevated in most cancer cells and is required for telomere length maintenance and immortalization of cancer cells. Glucose metabolic reprogramming is a hallmark of cancer and accompanied with increased expression of key… read more here.
Keywords: immortalization; cell; telomerase activity; activity ... See more keywords
Quercetin Interrupts the Positive Feedback Loop Between STAT3 and IL-6, Promotes Autophagy, and Reduces ROS, Preventing EBV-Driven B Cell Immortalization
Sign Up to like & getrecommendations! Published in 2019 at "Biomolecules"
DOI: 10.3390/biom9090482
Abstract: The oncogenic gammaherpesvirus Epstein–Barr virus (EBV) immortalizes in vitro B lymphocytes into lymphoblastoid cell lines (LCLs), a model that gives the opportunity to explore the molecular mechanisms driving viral tumorigenesis. In this study, we addressed… read more here.
Keywords: immortalization; cell immortalization; preventing ebv; ebv driven ... See more keywords
Immortalization Reversibility in the Context of Cell Therapy Biosafety
Sign Up to like & getrecommendations! Published in 2023 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms24097738
Abstract: Immortalization (genetically induced prevention of replicative senescence) is a promising approach to obtain cellular material for cell therapy or for bio-artificial organs aimed at overcoming the problem of donor material shortage. Immortalization is reversed before… read more here.
Keywords: immortalization; cell; cell therapy; immortalization reversibility ... See more keywords