Epitope Fine Mapping by Mass Spectrometry: Investigations of Immune Complexes Consisting of Monoclonal Anti‐HpTGEKP Antibody and Zinc Finger Protein Linker Phospho‐Hexapeptides
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200390
Abstract: Accurate formation of antibody‐antigen complexes has been relied on in both, multitudes of scientific projects and ample therapeutic and diagnostic applications. Mass spectrometrically determined dissociation behavior of immune complexes with the anti‐HpTGEKP antibody revealed that… read more here.
Keywords: immune complexes; linker; phospho; zinc finger ... See more keywords
A novel general and efficient technique for dissociating antigen in circulating immune complexes
Sign Up to like & getrecommendations! Published in 2018 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201700246
Abstract: Circulating immune complexes (CICs) are produced during the immune response. It is more clinically important to establish a general and efficient CICs dissociation technique for the detection of antigens for CICs other than the detection… read more here.
Keywords: technique; immune complexes; dissociation; precipitation ... See more keywords
Specific features of immune complexes in patients with sarcoidosis and pulmonary tuberculosis
Sign Up to like & getrecommendations! Published in 2018 at "Immunologic Research"
DOI: 10.1007/s12026-018-9052-1
Abstract: Clinical and radiological features of tuberculosis and sarcoidosis are quite overlapping, and therefore, a diagnostic dilemma often persists. There are no commonly accepted criteria for the diagnosis of sarcoidosis due to the lack of data… read more here.
Keywords: immune complexes; pulmonary tuberculosis; complexes patients; etiology ... See more keywords
Characterization of Large Immune Complexes with Size Exclusion Chromatography and Native Mass Spectrometry Supplemented with Gas Phase Ion Chemistry.
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DOI: 10.1021/acs.analchem.3c03278
Abstract: Large immune complexes formed by the cross-linking of antibodies with polyvalent antigens play critical roles in modulating cell-mediated immunity. While both the size and the shape of immune complexes are important determinants in Fc receptor-mediated… read more here.
Keywords: gas phase; chemistry; size; immune complexes ... See more keywords
Molecular architecture and platelet-activating properties of small immune complexes assembled on heparin and platelet factor 4
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DOI: 10.1038/s42003-024-05982-4
Abstract: Heparin-induced thrombocytopenia (HIT) is an adverse reaction to heparin leading to a reduction in circulating platelets with an increased risk of thrombosis. It is precipitated by polymerized immune complexes consisting of pathogenic antibodies that recognize… read more here.
Keywords: pf4; platelet; immune complexes; assembled heparin ... See more keywords
Making Weak Antigens Strong: Preparing Immune Complexes for Injection.
Sign Up to like & getrecommendations! Published in 2021 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot099978
Abstract: If antibodies against a particular antigen are available, that antigen can be purified and used for further immunizations, and antigens thus purified can show enhanced immunogenicity. Purified immune complexes can be injected directly, or while… read more here.
Keywords: immune complexes; strong preparing; antigens strong; weak antigens ... See more keywords
Identification and characterization of circulating immune complexes in IgA nephropathy
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DOI: 10.1126/sciadv.abm8783
Abstract: The underlying pathology of immunoglobulin A (IgA) nephropathy (IgAN), the most common glomerulonephritis worldwide, is driven by the deposition of immune complexes containing galactose-deficient IgA1 [Tn(+)IgA1] in the glomerular mesangium. Here, we report that novel… read more here.
Keywords: circulating immune; iga nephropathy; characterization circulating; immune complexes ... See more keywords
A root cause analysis to identify the mechanistic drivers of immunogenicity against the anti-VEGF biotherapeutic brolucizumab
Sign Up to like & getrecommendations! Published in 2023 at "Science Translational Medicine"
DOI: 10.1126/scitranslmed.abq5068
Abstract: Immunogenicity against intravitreally administered brolucizumab has been previously described and associated with cases of severe intraocular inflammation, including retinal vasculitis/retinal vascular occlusion (RV/RO). The presence of antidrug antibodies (ADAs) in these patients led to the… read more here.
Keywords: pharmacology; immune complexes; immunogenicity; mechanistic drivers ... See more keywords
Fcγ-receptor-IIIA bioactivity of circulating and synovial immune complexes in rheumatoid arthritis
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DOI: 10.1136/rmdopen-2024-004190
Abstract: Objective Previous technical limitations prevented the proof of Fcγ-receptor (FcγR)-activation by soluble immune complexes (sICs) in patients. FcγRIIIa (CD16) is a risk factor in rheumatoid arthritis (RA). We aimed at determining the presence of CD16-activating… read more here.
Keywords: bioactivity; immune complexes; rheumatoid arthritis; circulating sics ... See more keywords
Complement mediates binding and procoagulant effects of ultra-large HIT immune complexes.
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DOI: 10.1182/blood.2020009487
Abstract: Heparin-induced thrombocytopenia (HIT) is a prothrombotic disorder mediated by ultra-large immune complexes (ULICs) containing IgG antibodies to a multivalent antigen composed of platelet factor 4 (PF4) and heparin. The limitations of current anti-thrombotic therapy in… read more here.
Keywords: immune; immune complexes; complement mediates; ultra large ... See more keywords
Paraffin immunofluorescence for detection of immune complexes in renal biopsies: an efficient salvage technique for diagnosis of glomerulonephritis in dogs
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DOI: 10.1186/s12917-017-1287-x
Abstract: BackgroundRenal biopsy is an essential tool for the diagnosis of proteinuric kidney diseases in dogs, and evaluation of immune complexes (IC) by immunofluorescence (IF) of frozen sections (IF-F) is required for the diagnosis of IC-mediated… read more here.
Keywords: immune complexes; technique diagnosis; diagnosis; immunofluorescence ... See more keywords