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Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9853-1_16
Abstract: Fluorescence-activated cell sorting (FACS) in combination with yeast surface display has emerged as a vital tool for the isolation and engineering of antibodies and antibody-derived fragments from synthetic, naïve, and immune libraries. However, the generation…
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Keywords:
generation;
surface display;
immune libraries;
yeast surface ... See more keywords
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2
Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.794389
Abstract: Yeast-surface display (YSD) is commonly applied to screen Fab immune or naïve libraries for binders of predefined target molecules. However, reformatting of isolated variants represents a time-intensive bottleneck. Herein, we present a novel approach to…
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Keywords:
yeast surface;
surface display;
immune libraries;
antibody ... See more keywords