High‐throughput immunoaffinity enrichment and N‐glycan analysis of human plasma haptoglobin
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28280
Abstract: Haptoglobin (Hp) is a positive acute phase protein, synthesized in the liver, with four N‐glycosylation sites carrying mainly complex type N‐glycans. Its glycosylation is altered in different types of diseases but still has not been… read more here.
Keywords: high throughput; analysis; human plasma; immunoaffinity ... See more keywords
Simultaneous Determination of Ractopamine, Chloramphenicol, and Zeranols in Animal-Originated Foods by LC-MS/MS Analysis with Immunoaffinity Clean-up Column
Sign Up to like & getrecommendations! Published in 2017 at "Food Analytical Methods"
DOI: 10.1007/s12161-017-0858-6
Abstract: A novel analytical method employing immunoaffinity column (IAC) clean-up coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of ractopamine, chloramphenicol, and zeranols (α-zearalanol, β-zearalanol, zearalanone, α-zearalenol, β-zearalenol, and zearalenone) in… read more here.
Keywords: immunoaffinity; column; animal originated; ractopamine chloramphenicol ... See more keywords
Development of a Novel Monoclonal Antibody–Based Indirect Competitive ELISA with Immunoaffinity Cleanup for the Detection of Triclosan in Chickens
Sign Up to like & getrecommendations! Published in 2019 at "Food Analytical Methods"
DOI: 10.1007/s12161-019-01644-y
Abstract: Triclosan (TCS) was quantitatively analyzed for the first time in chicken samples using immunoaffinity cleanup followed by indirect competitive enzyme-linked immunosorbent assay (icELISA). Monoclonal antibodies (mAbs) against TCS were prepared. The mAb 6E1 showed high… read more here.
Keywords: detection; immunoaffinity; indirect competitive; immunoaffinity cleanup ... See more keywords
Chitosan microparticle-based immunoaffinity chromatography supports prepared by membrane emulsification technique: Characterization and application.
Sign Up to like & getrecommendations! Published in 2019 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2019.04.064
Abstract: In this study, chitosan microparticles intended to be used as matrix support for immunoaffinity chromatography were prepared by membrane emulsification technique. Regular spherical chitosan microparticles were obtained with an average diameter of 62.9μm and a… read more here.
Keywords: chitosan microparticles; immunoaffinity; column; immunoaffinity chromatography ... See more keywords
Depleting high-abundant and enriching low-abundant proteins in human serum: An evaluation of sample preparation methods using magnetic nanoparticle, chemical depletion and immunoaffinity techniques.
Sign Up to like & getrecommendations! Published in 2017 at "Talanta"
DOI: 10.1016/j.talanta.2017.03.091
Abstract: The efficiency of three different depletion methods to remove the most abundant proteins, enriching those human serum proteins with low abundance is checked to make more efficient the search and discovery of biomarkers. These methods… read more here.
Keywords: abundant proteins; immunoaffinity; low abundant; human serum ... See more keywords
Immunoaffinity chromatography combined with tandem mass spectrometry: A new tool for the selective capture and analysis of brassinosteroid plant hormones.
Sign Up to like & getrecommendations! Published in 2017 at "Talanta"
DOI: 10.1016/j.talanta.2017.04.044
Abstract: Brassinosteroids (BRs) are plant-specific steroid hormones that play essential roles in the regulation of many important physiological processes in plant life. Their extremely low concentrations (~pmoles/g FW) in plant tissue and huge differences in polarity… read more here.
Keywords: chromatography; immunoaffinity; plant; mass spectrometry ... See more keywords
Multiplexed Phosphoproteomic Profiling Using Titanium Dioxide and Immunoaffinity Enrichments Reveals Complementary Phosphorylation Events.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.6b00905
Abstract: A comprehensive view of protein phosphorylation remains an unmet challenge in the field of cell biology. Mass spectrometry-based proteomics is one of the most promising approaches for identifying thousands of phosphorylation events in a single… read more here.
Keywords: multiplexed phosphoproteomic; immunoaffinity; titanium dioxide; phosphorylation events ... See more keywords
Immunoaffinity Plastic Blade Spray Mass Spectrometry for Rapid Confirmatory Analysis of Food Contaminants
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.2c00149
Abstract: The lack of chromatographic separation in ambient and direct mass spectrometry (MS) ionization techniques jeopardizes the overall selectivity of the developed methods. Incorporating a biosensing element at the ionization source could compensate for that inherent… read more here.
Keywords: mass spectrometry; blade spray; blade; immunoaffinity ... See more keywords
A simple and high throughput parallel dual immunoaffinity liquid chromatography-mass spectrometry system for urine drug testing
Sign Up to like & getrecommendations! Published in 2018 at "Analytical Methods"
DOI: 10.1039/c7ay02755a
Abstract: A simple and rapid method for direct quantitation of drugs in human urine samples was developed using a system composed of an automatic column switch and two home-made capillary immunoaffinity columns (CIACs, 100 μm ×… read more here.
Keywords: system; immunoaffinity; column; system urine ... See more keywords
Development and application of an immunoaffinity column clean-up for enrofloxacin determination in food samples
Sign Up to like & getrecommendations! Published in 2017 at "Food and Agricultural Immunology"
DOI: 10.1080/09540105.2016.1263984
Abstract: ABSTRACT Enrofloxacin (ENR) is a synthetic fluoroquinolone antibiotic used for the treatment of infection in animals. The use of ENR has been strictly regulated because of its harmful effect on consumers of animal products. In… read more here.
Keywords: determination food; immunoaffinity column; immunoaffinity; column ... See more keywords
Development of an immunoaffinity chromatography and LC-MS/MS method for the determination of 6 zearalenones in animal feed
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0193584
Abstract: A novel and simple method for detecting 6 zearalenones in animal feed using liquid chromatography coupled with triple quadrupole mass spectrometry (LC-MS/MS) and immunoaffinity columns (IAC) was developed. The chromatographic peaks of the 6 zearalenones… read more here.
Keywords: chromatography; immunoaffinity; method; animal feed ... See more keywords