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Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0640-7_30
Abstract: Cerebral cavernous malformations (CCM) is a familial or sporadic rare disorder that is characterized by capillary vascular lesions with a mulberry-like appearance on MRI scans. Three distinct genes have been associated to CCM disease, known…
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Keywords:
p62 sqstm1;
immunofluorescence;
ccm disease;
detection p62 ... See more keywords
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Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1948-3_9
Abstract: Immunofluorescence is an important immunochemical technique that utilizes fluorescence-labeled antibodies to detect specific target antigens. It is used widely in both scientific research and clinical laboratories. Immunofluorescence allows for excellent sensitivity and amplification of signal…
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Keywords:
immunofluorescence;
biology;
fluorescent immunohistochemistry;
fluorescence ... See more keywords
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Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6673-8_14
Abstract: Immunofluorescence microscopy is an invaluable tool for the study of biological processes at the cellular level. While the localization of surface-exposed antigens can easily be determined using fluorescent antibodies, localization of intracellular antigens requires permeabilization…
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Keywords:
streptococcus pyogenes;
high resolution;
microscopy;
immunofluorescence ... See more keywords
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Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7144-2_1
Abstract: Circulating tumor cells (CTCs) are an important biomarker and their analysis can be considered a form of "liquid biopsy." The purpose of this book chapter is to describe the use of the 4-channel CMx (cells…
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Keywords:
tumor cells;
immunofluorescence;
circulating tumor;
cancer ... See more keywords
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Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8910-2_5
Abstract: Visualization of in vivo protein levels and localization is essential to analysis and elucidation of Hippo signaling mechanisms and its roles in diverse tissues. This is best done by imaging proteins using fluorescent labels. Fluorescent…
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Keywords:
hippo;
hippo signaling;
components drosophila;
signaling components ... See more keywords
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Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9012-2_5
Abstract: The cell lineage tracing system has been used predominantly in developmental biology studies. The Cre recombinase allows for the activation of the reporter in a specific cell line and all progeny. In this protocol, we…
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Keywords:
cell lineage;
lineage tracing;
immunofluorescence;
biology ... See more keywords
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Published in 2017 at "Surgical Endoscopy"
DOI: 10.1007/s00464-017-5638-2
Abstract: The ability to assess tissue perfusion and identify vital structures in the operating room in order to potentially decrease complication rates remains a key goal for surgeons. The introduction of immunofluorescence utilizing indocyanine green intraoperatively…
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Keywords:
surgery;
indocyanine green;
perfusion;
anatomy ... See more keywords
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Published in 2022 at "Journal of Nephrology"
DOI: 10.1007/s40620-022-01320-1
Abstract: estimated filtration glomerulitis, glomerulonephritis, immunoglobulin G, immunofluorescence, inflammation myeloperoxidase,
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Keywords:
testing indirect;
immunofluorescence;
glomerulonephritis;
panca autoantibody ... See more keywords
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Published in 2019 at "Journal of Neuroscience Methods"
DOI: 10.1016/j.jneumeth.2019.03.003
Abstract: BACKGROUND In the analysis of animal models of CNS diseases such as experimental autoimmune encephalomyelitis (EAE), immunostaining and histopathology are important readouts. However, the complex morphological features of a tissue staining are often reduced to…
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Keywords:
unsupervised quantification;
immunofluorescence;
analysis;
animal models ... See more keywords
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Published in 2020 at "Journal of Neuroimmunology"
DOI: 10.1016/j.jneuroim.2020.577415
Abstract: A marked prozone effect was observed in indirect immunofluorescence with human sera and human cerebrospinal fluid in two clinical cases involving breast carcinoma with paraneoplastic neuronal antibodies, and anti- N-methyl-D-aspartic acid (NMDA) receptor antibodies. Anti-Yo…
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Keywords:
indirect immunofluorescence;
prozone phenomenon;
immunofluorescence;
antibodies anti ... See more keywords
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Published in 2017 at "Journal of Endodontics"
DOI: 10.1016/j.joen.2017.02.001
Abstract: Introduction: The purpose of this study was to evaluate the in vivo response of periradicular tissues after sealing of furcation perforations with Biodentine, mineral trioxide aggregate (MTA), and gutta‐percha by means of histopathologic and indirect…
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Keywords:
biodentine;
response;
furcation;
tissue ... See more keywords