Articles with "immunofluorescence" as a keyword



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Detection of p62/SQSTM1 Aggregates in Cellular Models of CCM Disease by Immunofluorescence.

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Published in 2020 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-0640-7_30

Abstract: Cerebral cavernous malformations (CCM) is a familial or sporadic rare disorder that is characterized by capillary vascular lesions with a mulberry-like appearance on MRI scans. Three distinct genes have been associated to CCM disease, known… read more here.

Keywords: p62 sqstm1; immunofluorescence; ccm disease; detection p62 ... See more keywords
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Fluorescent Immunohistochemistry.

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Published in 2022 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-1948-3_9

Abstract: Immunofluorescence is an important immunochemical technique that utilizes fluorescence-labeled antibodies to detect specific target antigens. It is used widely in both scientific research and clinical laboratories. Immunofluorescence allows for excellent sensitivity and amplification of signal… read more here.

Keywords: immunofluorescence; biology; fluorescent immunohistochemistry; fluorescence ... See more keywords
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Detection of Intracellular Proteins by High-Resolution Immunofluorescence Microscopy in Streptococcus pyogenes.

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Published in 2017 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-6673-8_14

Abstract: Immunofluorescence microscopy is an invaluable tool for the study of biological processes at the cellular level. While the localization of surface-exposed antigens can easily be determined using fluorescent antibodies, localization of intracellular antigens requires permeabilization… read more here.

Keywords: streptococcus pyogenes; high resolution; microscopy; immunofluorescence ... See more keywords
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Microfluidic Capture and Multiplex Immunofluorescence of Circulating Tumor Cells to Identify Cancer of Origin.

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Published in 2017 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-7144-2_1

Abstract: Circulating tumor cells (CTCs) are an important biomarker and their analysis can be considered a form of "liquid biopsy." The purpose of this book chapter is to describe the use of the 4-channel CMx (cells… read more here.

Keywords: tumor cells; immunofluorescence; circulating tumor; cancer ... See more keywords
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Localization of Hippo Signaling Components in Drosophila by Fluorescence and Immunofluorescence.

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Published in 2019 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-8910-2_5

Abstract: Visualization of in vivo protein levels and localization is essential to analysis and elucidation of Hippo signaling mechanisms and its roles in diverse tissues. This is best done by imaging proteins using fluorescent labels. Fluorescent… read more here.

Keywords: hippo; hippo signaling; components drosophila; signaling components ... See more keywords
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Application of Cell Lineage Tracing Combined with Immunofluorescence in the Study of Dentinogenesis.

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Published in 2019 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-9012-2_5

Abstract: The cell lineage tracing system has been used predominantly in developmental biology studies. The Cre recombinase allows for the activation of the reporter in a specific cell line and all progeny. In this protocol, we… read more here.

Keywords: cell lineage; lineage tracing; immunofluorescence; biology ... See more keywords
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SAGES Technology and Value Assessment Committee safety and effectiveness analysis on immunofluorescence in the operating room for biliary visualization and perfusion assessment

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Published in 2017 at "Surgical Endoscopy"

DOI: 10.1007/s00464-017-5638-2

Abstract: The ability to assess tissue perfusion and identify vital structures in the operating room in order to potentially decrease complication rates remains a key goal for surgeons. The introduction of immunofluorescence utilizing indocyanine green intraoperatively… read more here.

Keywords: surgery; indocyanine green; perfusion; anatomy ... See more keywords
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pANCA autoantibody testing by indirect immunofluorescence indicates interstitial arteritis independent of MPO-ANCA immunoassays in ANCA-associated glomerulonephritis

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Published in 2022 at "Journal of Nephrology"

DOI: 10.1007/s40620-022-01320-1

Abstract: estimated filtration glomerulitis, glomerulonephritis, immunoglobulin G, immunofluorescence, inflammation myeloperoxidase, read more here.

Keywords: testing indirect; immunofluorescence; glomerulonephritis; panca autoantibody ... See more keywords
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Unsupervised quantification of tissue immunofluorescence in animal models of multiple sclerosis – Instructions for use

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Published in 2019 at "Journal of Neuroscience Methods"

DOI: 10.1016/j.jneumeth.2019.03.003

Abstract: BACKGROUND In the analysis of animal models of CNS diseases such as experimental autoimmune encephalomyelitis (EAE), immunostaining and histopathology are important readouts. However, the complex morphological features of a tissue staining are often reduced to… read more here.

Keywords: unsupervised quantification; immunofluorescence; analysis; animal models ... See more keywords
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Prozone phenomenon observed in indirect immunofluorescence assay by antibodies against neuronal antigens

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Published in 2020 at "Journal of Neuroimmunology"

DOI: 10.1016/j.jneuroim.2020.577415

Abstract: A marked prozone effect was observed in indirect immunofluorescence with human sera and human cerebrospinal fluid in two clinical cases involving breast carcinoma with paraneoplastic neuronal antibodies, and anti- N-methyl-D-aspartic acid (NMDA) receptor antibodies. Anti-Yo… read more here.

Keywords: indirect immunofluorescence; prozone phenomenon; immunofluorescence; antibodies anti ... See more keywords
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Furcation Perforation: Periradicular Tissue Response to Biodentine as a Repair Material by Histopathologic and Indirect Immunofluorescence Analyses

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Published in 2017 at "Journal of Endodontics"

DOI: 10.1016/j.joen.2017.02.001

Abstract: Introduction: The purpose of this study was to evaluate the in vivo response of periradicular tissues after sealing of furcation perforations with Biodentine, mineral trioxide aggregate (MTA), and gutta‐percha by means of histopathologic and indirect… read more here.

Keywords: biodentine; response; furcation; tissue ... See more keywords