Histology-Compatible MALDI Mass Spectrometry Based Imaging of Neuronal Lipids for Subsequent Immunofluorescent Staining.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b00313
Abstract: Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) enables acquisition of spatial distribution maps for molecular species in situ. This can provide comprehensive insights on the pathophysiology of different diseases. However, current sample preparation and MALDI-IMS… read more here.
Keywords: maldi ims; mass spectrometry; histology; neuronal lipids ... See more keywords
Immunofluorescent Staining of Whole-Mount Mouse Embryos.
Sign Up to like & getrecommendations! Published in 2018 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot094037
Abstract: Mouse embryos and fetal organs have no pigmentation except for the retinal pigmented epithelium of the eye at subsequent stages of development. This makes it difficult to visualize and photodocument embryonic structures using conventional light… read more here.
Keywords: whole mount; microscopy; staining whole; mouse embryos ... See more keywords
Cover Image: Invasion of a cutaneous melanoma tumour
Sign Up to like & getrecommendations! Published in 2017 at "British Journal of Dermatology"
DOI: 10.1111/bjd.15675
Abstract: DEAR EDITOR, This image illustrates the vertical cutaneous invasion of an early-stage melanoma tumour. Human melanoma cells were identified by immunofluorescent staining of Melan-A, a marker of melanocytic lineage (red), within the cutaneous microenvironment. These… read more here.
Keywords: melanoma; invasion; melanoma tumour; cover image ... See more keywords
A new approach to epithelial-mesenchymal transition diagnostics in epithelial tumors: double immunofluorescent staining and flow cytometry.
Sign Up to like & getrecommendations! Published in 2020 at "BioTechniques"
DOI: 10.2144/btn-2020-0024
Abstract: A new method of double immunofluorescent staining for flow cytometry has been created to evaluate quantitative expression of mesenchymal protein vimentin only in epithelial cells of a solid tumor that is a mix of different origin… read more here.
Keywords: mesenchymal transition; double immunofluorescent; epithelial mesenchymal; immunofluorescent staining ... See more keywords
Protecting RNA quality for spatial transcriptomics while improving immunofluorescent staining quality
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Neuroscience"
DOI: 10.3389/fnins.2023.1198154
Abstract: In comparison to bulk sequencing or single cell sequencing, spatial transcriptomics preserves the spatial information in tissue slices and can even be mapped to immunofluorescent stainings, allowing translation of gene expression information into their spatial… read more here.
Keywords: quality; immunofluorescent staining; protecting rna; quality spatial ... See more keywords