Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy
Sign Up to like & getrecommendations! Published in 2020 at "Cancer Communications"
DOI: 10.1002/cac2.12023
Abstract: Conventional immunohistochemistry (IHC) is a widely used diagnostic technique in tissue pathology. However, this technique is associated with a number of limitations, including high inter‐observer variability and the capacity to label only one marker per… read more here.
Keywords: immunohistochemistry; immunohistochemistry immunofluorescence; multiplex immunohistochemistry; era cancer ... See more keywords
Using immunohistochemistry to classify the molecular subtypes of Paget's disease of the breast.
Sign Up to like & getrecommendations! Published in 2023 at "Cancer medicine"
DOI: 10.1002/cam4.6066
Abstract: PURPOSE To classify the molecular subtypes of Paget's disease of the breast, and then compare them with general breast cancer to get deeper understanding of this disease and offer better management of associate patients in… read more here.
Keywords: molecular subtypes; immunohistochemistry; disease; breast ... See more keywords
Pleural metastasis from parotid secretory carcinoma: First report of morphology on effusion cytology, and role of pan‐TRK immunohistochemistry
Sign Up to like & getrecommendations! Published in 2022 at "Diagnostic Cytopathology"
DOI: 10.1002/dc.25057
Abstract: Distant metastasis from salivary gland secretory carcinoma (SC) is rare, with lung and pleura being the most frequent site. While cytological features of SC on fine needle aspirates are well documented, its morphology in serous… read more here.
Keywords: immunohistochemistry; secretory carcinoma; effusion cytology; pan trk ... See more keywords
N‐terminus DUX4‐immunohistochemistry is a reliable methodology for the diagnosis of DUX4‐fused B‐lymphoblastic leukemia/lymphoma (N‐terminus DUX4 IHC for DUX4‐fused B‐ALL)
Sign Up to like & getrecommendations! Published in 2022 at "Genes"
DOI: 10.1002/gcc.23033
Abstract: B‐lymphoblastic leukemia/lymphoma (B‐ALL) is the most common pediatric malignancy and the most commonly diagnosed adult lymphoblastic leukemia. Recent advances have broadened the spectrum of B‐ALL, with DUX4 gene fusions implicated in a subclass occurring in… read more here.
Keywords: terminus dux4; immunohistochemistry; methodology; dux4 ... See more keywords
Glycoform-Specific Visualization in Immunohistochemistry by "Lectin Inhibition".
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DOI: 10.1007/978-1-0716-0430-4_18
Abstract: Antibodies are useful for localizing glycoprotein antigens in histochemistry, but they do not differentiate glycoforms in tissue sections because conventional antibodies recognize only protein epitopes rather than glycans. Glycan epitopes are recognized by lectins, which… read more here.
Keywords: immunohistochemistry; lectin inhibition; glycoform specific; inhibition ... See more keywords
Western Blot as a Support Technique for Immunohistochemistry to Detect Programmed Cell Death Ligand 1 Expression.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1278-1_5
Abstract: Antibody selection and optimization are crucial to guarantee accurate and reproducible results when using such antibodies for applications such as western blot analysis and immunohistochemistry (IHC). This is especially important when selecting good candidate antibodies… read more here.
Keywords: technique; immunohistochemistry; western blot; programmed cell ... See more keywords
UltraPlex Hapten-Based Multiplexed Fluorescent Immunohistochemistry.
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DOI: 10.1007/978-1-0716-1593-5_17
Abstract: The UltraPlex method for multiplexed two-dimensional fluorescent immunohistochemistry is described, in which hapten tags conjugated to primary antibodies facilitate multiplexed imaging of four or more antigens per tissue section at once. Anti-hapten secondary antibodies labeled… read more here.
Keywords: ultraplex hapten; immunohistochemistry; tissue samples; hapten based ... See more keywords
Immunohistochemistry in Historical Perspective: Knowing the Past to Understand the Present.
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DOI: 10.1007/978-1-0716-1948-3_2
Abstract: Immunohistochemistry is an extraordinary and extensively used technique whereby antibodies are used to detect antigens in cells within a tissue section. It has numerous applications in medicine, particularly in cancer diagnosis. It was Albert Hewett… read more here.
Keywords: immunohistochemistry historical; immunohistochemistry; perspective knowing; knowing past ... See more keywords
Loss of H3K27 trimethylation by immunohistochemistry is frequent in oligodendroglioma, IDH-mutant and 1p/19q-codeleted, but is neither a sensitive nor a specific marker
Sign Up to like & getrecommendations! Published in 2020 at "Acta Neuropathologica"
DOI: 10.1007/s00401-019-02123-8
Abstract: The integrated diagnosis for infiltrating gliomas requires demonstration of IDH1 or IDH2 mutation and 1p/19q codeletion in the tumor to diagnose oligodendroglioma or anaplastic oligodendroglioma, IDH-mutant and 1p/19q-codeleted. IDH1 R132H, ATRX and p53 immunohistochemistry may… read more here.
Keywords: immunohistochemistry; gliomas; idh mutant; h3k27me3 ... See more keywords
Characteristics of the tissue section that influence the staining outcome in immunohistochemistry
Sign Up to like & getrecommendations! Published in 2018 at "Histochemistry and Cell Biology"
DOI: 10.1007/s00418-018-1742-1
Abstract: Immunohistochemistry (IHC) is influenced by several factors such as cold ischemia time, fixative, fixation time, paraffin, storage time, antibody, antigen retrieval technique and detection systems. In the setting of post-mortem tissue, not only post-mortem delay,… read more here.
Keywords: methodology; immunohistochemistry; outcome; tissue ... See more keywords
Book Review—Diagnostic immunohistochemistry, 5th edition
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DOI: 10.1007/s00428-018-2375-4
Abstract: BDo we still need Textbooks? After all, it is common practice to solve everyday’s pressing problems through dedicated websites.^ This is the first, basic question which comes to mind when dealing with BDabbs’ Immunohistochemistry,^ now… read more here.
Keywords: 5th edition; immunohistochemistry; pathology; book ... See more keywords