Immunoprecipitation Analyses of Estrogen Receptor α Phosphorylated at Serine 216 in the Mouse Liver.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1920-9_3
Abstract: Estrogen receptor α (ERα) conserves a phosphorylation motif at Serine 216. This site constitutes a protein kinase C phosphorylation motif located within the DNA binding domain (DBD) of ERα. The liver plays a critical role… read more here.
Keywords: immunoprecipitation; serine 216; phosphorylated serine; liver ... See more keywords
Analysis of Ethylene Receptor Interactions by Co-immunoprecipitation Assays.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6854-1_9
Abstract: Ethylene receptors are predominantly localized to the endoplasmic reticulum (ER) membrane, and coordinate ethylene signal output through protein-protein interactions with each other and additional signaling components. Here, we describe a co-immunoprecipitation (Co-IP) assay based on… read more here.
Keywords: receptor interactions; ethylene; analysis ethylene; ethylene receptors ... See more keywords
Quantification of RNA Content in Reconstituted Ebola Virus Nucleocapsids by Immunoprecipitation.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7116-9_7
Abstract: Immunoprecipitations are commonly used to isolate proteins or protein complexes and assess protein-protein interactions; however, they can also be used to assess protein-RNA complexes. Here we describe an adapted RNA immunoprecipitation technique that permits the… read more here.
Keywords: ebola virus; virus nucleocapsids; rna content; quantification rna ... See more keywords
Development of an antibody control system using phage display.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2019.05.009
Abstract: While chromatin immunoprecipitation has become a widely-used method in the field of transcription regulation studies, serious limitations connected to the complexity and relatively little standardization of the method serve as obstacles for its use in… read more here.
Keywords: control; chromatin immunoprecipitation; phage display; immunoprecipitation ... See more keywords
Quantifying Protein-Specific N-glycome Profiles by Focused Protein- and Immunoprecipitation-Glycomics.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.9b00232
Abstract: Serum N-glycans have been reported to be potential diagnostic and therapeutic biomarkers for many diseases and conditions, such as inflammation, fibrosis, and cancer progression. We previously described a method for glycomic analysis of gel-separated serum… read more here.
Keywords: specific glycome; glycome profiles; quantifying protein; immunoprecipitation glycomics ... See more keywords
Highly Specific Protein Identification by Immunoprecipitation–Mass Spectrometry Using Antifouling Microbeads
Sign Up to like & getrecommendations! Published in 2022 at "ACS Applied Materials & Interfaces"
DOI: 10.1021/acsami.1c22734
Abstract: A common method to study protein complexes is immunoprecipitation (IP), followed by mass spectrometry (thus labeled: IP-MS). IP-MS has been shown to be a powerful tool to identify protein–protein interactions. It is, however, often challenging… read more here.
Keywords: specific protein; highly specific; mass spectrometry; protein ... See more keywords
What to do about those immunoprecipitation blues
Sign Up to like & getrecommendations! Published in 2019 at "Nature Methods"
DOI: 10.1038/s41592-019-0365-3
Abstract: ChIP-seq, DIP-seq and related techniques are informative genome-wide assays, but they don’t always work as planned. read more here.
Keywords: immunoprecipitation blues; immunoprecipitation;
A Multianalyte Assay for the Detection of Dermatomyositis-Related Autoantibodies Based on Immunoprecipitation Combined With Immunoblotting.
Sign Up to like & getrecommendations! Published in 2022 at "Modern rheumatology"
DOI: 10.1093/mr/roac056
Abstract: OBJECTIVE To develop a multianalyte assay for the detection of dermatomyositis (DM)-related autoantibodies using immunoprecipitation (IP) combined with immunoblotting (IB). METHODS Sera from 116 DM patients were subjected to RNA and protein immunoprecipitation assays as… read more here.
Keywords: assay detection; related autoantibodies; anti; multianalyte assay ... See more keywords
Large-Scale Immunoprecipitation from Fission Yeast Cell Extracts.
Sign Up to like & getrecommendations! Published in 2017 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot091595
Abstract: We outline immunoprecipitation (IP) procedures to isolate the large quantities of a molecule of interest that are required to identify posttranslational modifications (PTMs) in subsequent targeted mass spectrometry analysis. In situ denaturation by trichloroacetic acid… read more here.
Keywords: large scale; immunoprecipitation fission; scale immunoprecipitation; yeast cell ... See more keywords
Immunoprecipitation and SDS-PAGE for Cross-Linking Immunoprecipitation (CLIP).
Sign Up to like & getrecommendations! Published in 2018 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot097956
Abstract: This first part of this protocol is designed to optimize purification of the RNABP by immunoprecipitation for cross-linking immunoprecipitation (CLIP) experiments. The key variables to assess are the quality and quantity of antibody needed to… read more here.
Keywords: rna; protocol; cross linking; clip ... See more keywords
Hybridoma Screening by Antigen Capture: Immunoprecipitation.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot103143
Abstract: Immunoprecipitation is rarely used for screening hybridoma fusions because the assays are tedious and time-consuming. However, it can be useful when working with complex antigens because the precipitated antigen is normally detected after sodium dodecyl… read more here.
Keywords: antigen capture; hybridoma screening; capture immunoprecipitation; screening antigen ... See more keywords