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   Articles with "immunosorbent assay" as a keyword



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Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay.

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recommendations! Published in 2021 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-1016-9_5

Abstract: Enzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for… read more here.

Keywords: c3a; linked immunosorbent; immunosorbent assay; complement activation ... See more keywords
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An enzyme-linked immunosorbent assay for the detection of diacetyl (2,3-butanedione).

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recommendations! Published in 2017 at "Analytical biochemistry"

DOI: 10.1016/j.ab.2017.07.021

Abstract: Diacetyl (2,3-butanedione) is an important metabolic marker of several cancers, as well as an important off-flavour component produced during fermentation. As a small molecule in a complex mixture with many other analytes, existing methods for… read more here.

Keywords: linked immunosorbent; diacetyl; immunosorbent assay; diacetyl butanedione ... See more keywords
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An indirect competitive enzyme-linked immunosorbent assay toward the standardization of Pueraria candollei based on its unique isoflavonoid, kwakhurin.

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recommendations! Published in 2019 at "Fitoterapia"

DOI: 10.1016/j.fitote.2018.12.010

Abstract: Kwakhurin (Kwa) is a plant secondary metabolite solely present in Pueraria candollei var. mirifica (P. candollei), which has long been used as a Thai traditional herb for estrogen replacement therapy. Recently, health hazards have arisen… read more here.

Keywords: mab 11f; standardization; linked immunosorbent; immunosorbent assay ... See more keywords
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Quantum dot‐linked immunosorbent assay (QLISA) using orientation‐directed antibodies

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recommendations! Published in 2017 at "Journal of Pharmaceutical and Biomedical Analysis"

DOI: 10.1016/j.jpba.2017.05.014

Abstract: &NA; An approach similar to the enzyme‐linked immunosorbent assay (ELISA), with the advantage of saving time and effort but exhibiting high performance, was developed using orientation‐directed half‐part antibodies immobilized on CdSe/ZnS quantum dots. ELISA is… read more here.

Keywords: linked immunosorbent; immunosorbent assay; quantum dots; quantum dot ... See more keywords
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Preparation of monoclonal antibody and development of an enzyme-linked immunosorbent assay for the detection of ceftiofur in animal-derived foods.

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recommendations! Published in 2021 at "Journal of pharmaceutical and biomedical analysis"

DOI: 10.1016/j.jpba.2021.114378

Abstract: Ceftiofur (CEF) residues in animal-derived foods are of great concern to farmers, regulatory agencies and consumers. In this study, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method was established to quickly monitor CEF residues in… read more here.

Keywords: derived foods; monoclonal antibody; linked immunosorbent; animal derived ... See more keywords
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Diagnostic accuracy of enzyme-linked immunosorbent assay (ELISA) and immunoblot (IB) for the detection of antibodies against Neospora caninum in milk from dairy cows

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recommendations! Published in 2017 at "Epidemiology and Infection"

DOI: 10.1017/s0950268817002916

Abstract: Abstract The aim of the study was to estimate the sensitivity and specificity of enzyme-linked immunosorbent assay (ELISA) and immunoblot (IB) for detecting antibodies of Neospora caninum in dairy cows, in the absence of a… read more here.

Keywords: assay elisa; linked immunosorbent; accuracy; elisa ... See more keywords
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A Multiple-Target Simultaneous Detection Method for Immunosorbent Assay and Immunospot Assay.

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recommendations! Published in 2022 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.2c01087

Abstract: Enzyme-linked immunosorbent assay (ELISA) is one of the most common methods in biological studies, and enzyme-linked immunospot (ELISpot) is a method to measure specific cell numbers by detecting protein secretion at a single-cell level. However,… read more here.

Keywords: immunospot; detection; folisa folispot; method ... See more keywords
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Double-Enzyme Active Vanadium Nanospheres-Mediated Ratiometric Multicolor Immunosensors for Sensitive Detection of the T-2 Toxin.

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recommendations! Published in 2023 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.2c05197

Abstract: Owing to its high throughput, simplicity, and rapidity, enzyme-linked immunosorbent assay (ELISA) has attracted much attention in the field of immunoassays. However, the traditional ELISA usually affords a single signal readout and the labeling ability… read more here.

Keywords: detection toxin; sensitive detection; detection; linked immunosorbent ... See more keywords
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Enzyme-Linked Immunosorbent Assay-Based Microarray on a Chip for Bioaerosol Sensing: Toward Sensitive and Multiplexed Profiling of Foodborne Allergens.

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recommendations! Published in 2023 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.3c00591

Abstract: Food allergy has become a growing health concern that may impair life quality and even cause life-threatening outcomes. Accidental and continuous exposure to allergenic bioaerosols has a substantially negative impact on the respiratory health of… read more here.

Keywords: foodborne allergens; food; enzyme linked; linked immunosorbent ... See more keywords
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A Versatile PdRu Bimetallic Nanoenzyme-Integrated Enzyme-Linked Immunosorbent Assay for Highly Sensitive Escherichia coli O157:H7 Detection.

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recommendations! Published in 2023 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.3c00743

Abstract: Nanozymes have drawn much attention as an enzyme mimetic with low cost and stability in enhancing analytical performance. Herein, a peroxidase-mimicking nanozyme-improved enzyme-linked immunosorbent assay (ELISA) was developed employing the bimetallic PdRu nanozyme to replace… read more here.

Keywords: pdru; enzyme linked; coli o157; detection ... See more keywords
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Development of a Nanobody-AviTag Fusion Protein and Its Application in a Streptavidin-Biotin-Amplified Enzyme-Linked Immunosorbent Assay for Ochratoxin A in Cereal.

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recommendations! Published in 2018 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.8b03085

Abstract: Ochratoxin A (OTA) is a common food contaminant that threatens consumers' safety and health. A sensitive and selective biotin-streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) for OTA using a nanobody-AviTag fusion protein (Nb-AviTag) was developed in this… read more here.

Keywords: avitag; linked immunosorbent; amplified enzyme; immunosorbent assay ... See more keywords