Altering the central carbon metabolism of HEK293 cells: Impact on recombinant glycoprotein quality.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2016.12.003
Abstract: The accumulation of metabolic by-products remains a critical challenge in the development of mammalian cells culture processes as it impacts cellular growth, productivity and product quality. Although the overexpression of the PYC2 gene was shown… read more here.
Keywords: impact recombinant; quality; metabolism; cells impact ... See more keywords
Proteomics and metabolic burden analysis to understand the impact of recombinant protein production in E. coli
Sign Up to like & getrecommendations! Published in 2024 at "Scientific Reports"
DOI: 10.1038/s41598-024-63148-y
Abstract: The impact of recombinant protein production (RPP) on host cells and the metabolic burden associated with it undermine the efficiency of the production system. This study utilized proteomics to investigate the dynamics of parent and… read more here.
Keywords: protein; metabolic burden; impact recombinant; protein production ... See more keywords