Improved dsDNA recombineering enables versatile multiplex genome engineering of kilobase-scale sequences in diverse bacteria
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DOI: 10.1093/nar/gkab1076
Abstract: Abstract Recombineering assisted multiplex genome editing generally uses single-stranded oligonucleotides for site directed mutational changes. It has proven highly efficient for functional screens and to optimize microbial cell factories. However, this approach is limited to… read more here.
Keywords: improved dsdna; multiplex genome; genome; genome engineering ... See more keywords