Context and number of noncanonical repeat variable diresidues impede the design of TALE proteins with improved DNA targeting
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DOI: 10.1002/pro.3801
Abstract: Transcription activator‐like effector (TALE) proteins have been used extensively for targeted binding of fusion proteins to loci of interest in (epi)genome engineering. Such approaches typically utilize four canonical TALE repeat variable diresidue (RVD) types, corresponding… read more here.
Keywords: tale proteins; tale; repeat variable; improved specificity ... See more keywords
Rationally Designed APOBEC3B Cytosine Base Editors with Improved Specificity.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular cell"
DOI: 10.1016/j.molcel.2020.07.005
Abstract: Cytosine base editors (CBEs) generate C-to-T nucleotide substitutions in genomic target sites without inducing double-strand breaks. However, CBEs such as BE3 can cause genome-wide off-target changes via sgRNA-independent DNA deamination. By leveraging the orthogonal R-loops… read more here.
Keywords: base editors; target; base; cytosine base ... See more keywords