Computer-Aided Targeted Mutagenesis of Thermoclostridium caenicola d-Allulose 3-Epimerase for Improved Thermostability.
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DOI: 10.1021/acs.jafc.1c07256
Abstract: d-Allulose 3-epimerase (DAEase) is a key enzyme in d-allulose bioproduction. DAEase from Thermoclostridium caenicola suffers from poor thermostability, hampering its large-scale applications in industry. In this study, mutants A70P, G107P, F155Y, and D162T with increased… read more here.
Keywords: improved thermostability; thermoclostridium caenicola; allulose epimerase; targeted mutagenesis ... See more keywords