Improving the mutagenesis efficiency of the Kunkel method by codon optimization and annealing temperature adjustment.
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DOI: 10.1016/j.nbt.2019.11.004
Abstract: The Kunkel method is a widely used site-directed mutagenesis strategy that introduces point mutations by annealing mutation-containing oligonucleotides to single-stranded uracil-containing DNA (dU-ssDNA) templates. The method is fast and inexpensive and has been routinely employed… read more here.
Keywords: mutagenesis; point mutations; kunkel method; improving mutagenesis ... See more keywords