Improving the thermostability of a GH97 dextran glucosidase by rational design
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology Letters"
DOI: 10.1007/s10529-020-02928-8
Abstract: This study was aimed at improving the thermostability of dextran glucosidase PspAG97A, a member of the glycoside hydrolase family 97, from Pseudoalteromonas sp. K8. A total of 9 lysine residues were chosen using the TKSA-MC… read more here.
Keywords: k75e k363e; improving thermostability; thermostability; charge ... See more keywords
Improving the Thermostability and Catalytic Activity of an Inulosucrase by Rational Engineering for the Biosynthesis of Microbial Inulin.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.1c04852
Abstract: Thermostability and enzymatic activity are two vital indexes determining the application of an enzyme on an industrial scale. A truncated inulosucrase, Laga-ISΔ138-702, from Lactobacillus gasseri showed high catalysis activity. To further enhance its thermostability and… read more here.
Keywords: improving thermostability; thermostability catalytic; thermostability; activity inulosucrase ... See more keywords
Improving Thermostability of Chimeric Enzymes Generated by Domain Shuffling Between Two Different Original Glucoamylases
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.881421
Abstract: In order to improve enzymatic properties of glucoamylases, six recombinant genes GA1–GA6 were created by domain shuffling of glucoamylase genes GAA1 from Aspergillus niger Ld418AI and GATE from Talaromyces emersonii Ld418 TE using overlap extension… read more here.
Keywords: thermostability chimeric; domain shuffling; ga1 ga2; gate ... See more keywords