Particulate Vaccines: Design of Bacterial Inclusion Bodies as Antigen Carrier Systems (Adv. Biosys. 11/2018)
Sign Up to like & getrecommendations! Published in 2018 at "Advanced Biosystems"
DOI: 10.1002/adbi.201870103
Abstract: co-workers show how bioengineering of mycobacterial antigens enhances their intermolecular interactions that mediate self-assembly of discrete antigen particles, inside a safe bacterial cell factory, Escherichia coli. The resulting pure antigen particles induce strong antigen-specific humoral… read more here.
Keywords: inclusion bodies; bacterial inclusion; particulate vaccines; vaccines design ... See more keywords
SFTS bunyavirus NSs protein sequestrates mTOR into inclusion bodies and deregulates mTOR‐ULK1 signaling, provoking pro‐viral autophagy
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28371
Abstract: Autophagy is emerging as a critical player in host defense against diverse infections, in addition to its conserved function to maintain cellular homeostasis. Strikingly, some pathogens have evolved strategies to evade, subvert or exploit different… read more here.
Keywords: mtor ulk1; viral autophagy; pro viral; inclusion bodies ... See more keywords
Nondenaturing Solubilization of Inclusion Bodies from Lactic Acid Bacteria.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1859-2_23
Abstract: Since inclusion bodies (IBs) contain an important amount of properly folded and active proteins, their solubilization using nondenaturing conditions to obtain aggregation-prone proteins has gained interest. Through these conditions, the refolding step is no longer… read more here.
Keywords: inclusion; inclusion bodies; bodies lactic; nondenaturing solubilization ... See more keywords
Purification of Inclusion Bodies Produced in Bacteria and Yeast.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1859-2_24
Abstract: Purification of inclusion bodies (IBs) is gaining importance due to the raising of novel applications for these submicron particulate protein clusters, with potential uses in the biomedical and biotechnological fields among others. Here, we present… read more here.
Keywords: bodies produced; inclusion bodies; bacteria yeast; purification inclusion ... See more keywords
Quality Control of Proteins Solubilized from Inclusion Bodies.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1859-2_28
Abstract: Despite substantial development of production and purification protocols for heterologous recombinant proteins, some proteins are difficult to produce or, when produced, are accumulated in inclusion bodies (IBs). Nondenaturing protocols can be used to recover the… read more here.
Keywords: inclusion; inclusion bodies; proteins solubilized; quality control ... See more keywords
Catalytically-active inclusion bodies for biotechnology—general concepts, optimization, and application
Sign Up to like & getrecommendations! Published in 2020 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-020-10760-3
Abstract: Bacterial inclusion bodies (IBs) have long been considered as inactive, unfolded waste material produced by heterologous overexpression of recombinant genes. In industrial applications, they are occasionally used as an alternative in cases where a protein… read more here.
Keywords: inclusion bodies; catalytically active; inclusion; catib ... See more keywords
Molecular chaperone HSP70 prevents formation of inclusion bodies of the 25-kDa C-terminal fragment of TDP-43 by preventing aggregate accumulation
Sign Up to like & getrecommendations! Published in 2018 at "Cell Stress and Chaperones"
DOI: 10.1007/s12192-018-0930-1
Abstract: Transactive response DNA/RNA-binding protein 43-kDa (TDP-43) C-terminal fragments, such as a 25-kDa fragment (TDP-25), have been identified as a ubiquitinated and phosphorylated components of inclusion bodies (IBs) in motor neurons from amyotrophic lateral sclerosis patients.… read more here.
Keywords: inclusion bodies; hsp70; ibs; tdp ... See more keywords
Efficient renaturation of inclusion body proteins denatured by SDS.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2017.07.003
Abstract: Inclusion bodies are often formed when the foreign protein is over expressed in Escherichia coli. Since proteins in inclusion bodies are inactive, denaturing and refolding of inclusion body proteins are necessary to obtain the active… read more here.
Keywords: inclusion bodies; inclusion; body proteins; sds ... See more keywords
Solubilization and renaturation of biologically active human bone morphogenetic protein-4 from inclusion bodies
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Reports"
DOI: 10.1016/j.btre.2018.e00249
Abstract: Highlights • Biologically active rhBMP-4 was produced in a prokaryotic host as inclusion bodies.• Different refolding recipes were tested for optimal dimerization yield.• One-step purification of dimer with cation-exchange membrane chromatography.• The product induces trophoblast… read more here.
Keywords: inclusion bodies; renaturation biologically; biologically active; active human ... See more keywords
Downstream processing of Cry4AaCter-induced inclusion bodies containing insect-derived antimicrobial peptides produced in Escherichia coli.
Sign Up to like & getrecommendations! Published in 2019 at "Protein expression and purification"
DOI: 10.1016/j.pep.2018.12.002
Abstract: The Cry4AaCter tag is a pull-down tag which promotes the formation of inclusion bodies (IBs) that can be resolubilized in an alkaline buffer. Here, we used the Cry4AaCter tag to create a platform for the… read more here.
Keywords: inclusion bodies; escherichia coli; impi i38v; antimicrobial peptides ... See more keywords
Refolding, purification, and characterization of constitutive-active human-Smad8 produced as inclusion bodies in ClearColi® BL21 (DE3).
Sign Up to like & getrecommendations! Published in 2021 at "Protein expression and purification"
DOI: 10.1016/j.pep.2021.105878
Abstract: Smad8 is a transcriptional regulator that participates in the intracellular signaling pathway of the transforming growth factor-β (TGF-β) family. Full-length Smad8 is an inactive protein in the absence of ligand stimulation. The expression of a… read more here.
Keywords: inclusion bodies; bl21 de3; smad8; clearcoli bl21 ... See more keywords