Increasing the buffering capacity of minimal media leads to higher protein yield
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DOI: 10.1007/s10858-018-00222-4
Abstract: We describe a general and simple modification to the standard M9 minimal medium recipe that leads to an approximate twofold increase in the yield of heterologously expressed proteins in Escherichia coli BL21(DE3) bacteria. We monitored… read more here.
Keywords: capacity minimal; protein yield; increasing buffering; yield ... See more keywords