Increasing the specificity of CRISPR systems with engineered RNA secondary structures
Sign Up to like & getrecommendations! Published in 2019 at "Nature biotechnology"
DOI: 10.1038/s41587-019-0095-1
Abstract: CRISPR (clustered regularly interspaced short palindromic repeat) systems have been broadly adopted for basic science, biotechnology, and gene and cell therapy. In some cases, these bacterial nucleases have demonstrated off-target activity. This creates a potential… read more here.
Keywords: rna secondary; crispr systems; crispr; increasing specificity ... See more keywords
Increasing Specificity of Targeted DNA Methylation Editing by Non-Enzymatic CRISPR/dCas9-Based Steric Hindrance
Sign Up to like & getrecommendations! Published in 2023 at "Biomedicines"
DOI: 10.3390/biomedicines11051238
Abstract: As advances in genome engineering inch the technology towards wider clinical use—slowed by technical and ethical hurdles—a newer offshoot, termed “epigenome engineering”, offers the ability to correct disease-causing changes in the DNA without changing its… read more here.
Keywords: methylation editing; increasing specificity; dna methylation; targeted dna ... See more keywords