Integration of data-independent acquisition (DIA) with co-fractionation mass spectrometry (CF-MS) to enhance interactome mapping capabilities.
Sign Up to like & getrecommendations! Published in 2023 at "Proteomics"
DOI: 10.1002/pmic.202200278
Abstract: Proteomics technologies are continually advancing, providing opportunities to develop stronger and more robust protein interaction networks (PINs). In part, this is due to the ever-growing number of high-throughput proteomics methods that are available. This review… read more here.
Keywords: data independent; dia; dia fractionation; acquisition dia ... See more keywords
Label-Free Proteomics of Quantity-Limited Samples Using Ion Mobility-Assisted Data-Independent Acquisition Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1024-4_23
Abstract: Over the past two decades, unbiased data-independent acquisition (DIA) approaches have gained increasing popularity in the bottom-up proteomics field. Here, we describe an ion mobility separation enhanced DIA workflow for large-scale label-free quantitative proteomics studies… read more here.
Keywords: data independent; limited samples; independent acquisition; ion mobility ... See more keywords
Mapping Biological Networks from Quantitative Data-Independent Acquisition Mass Spectrometry: Data to Knowledge Pipelines.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6783-4_19
Abstract: Data-independent acquisition mass spectrometry (DIA-MS) strategies and applications provide unique advantages for qualitative and quantitative proteome probing of a biological sample allowing constant sensitivity and reproducibility across large sample sets. These advantages in LC-MS/MS are… read more here.
Keywords: data independent; mass spectrometry; data knowledge; knowledge ... See more keywords
CSF Sample Preparation for Data-Independent Acquisition.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9706-0_4
Abstract: To study changes in neurological diseases and to identify disease-related mechanisms or biomarkers for diagnosis, cerebrospinal fluid (CSF) is frequently used for proteomic-based discovery. In the last years, development and application of mass spectrometry (MS)… read more here.
Keywords: data independent; analysis; independent acquisition; csf ... See more keywords
Improving Precursor Selectivity in Data-Independent Acquisition Using Overlapping Windows
Sign Up to like & getrecommendations! Published in 2019 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1007/s13361-018-2122-8
Abstract: AbstractA major goal of proteomics research is the accurate and sensitive identification and quantification of a broad range of proteins within a sample. Data-independent acquisition (DIA) approaches that acquire MS/MS spectra independently of precursor information… read more here.
Keywords: dia; data independent; precursor selectivity; independent acquisition ... See more keywords
Comprehensive MS/MS profiling by UHPLC-ESI-QTOF-MS/MS using SWATH data-independent acquisition for the study of platelet lipidomes in coronary artery disease.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2018.08.060
Abstract: A non-targeted lipidomics workflow based on C8 core-shell particle ultra high-performance liquid chromatography (UHPLC) hyphenated to ESI-QTOF-MS in data-independent acquisition (DIA) mode with sequential window acquisition of all theoretical fragment ion spectra (SWATH) was developed… read more here.
Keywords: data independent; swath; independent acquisition; acquisition ... See more keywords
Development of a data independent acquisition mass spectrometry workflow to enable glycopeptide analysis without predefined glycan compositional knowledge.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2017.10.011
Abstract: Glycoproteomics investigates glycan moieties in a site specific manner to reveal the functional roles of protein glycosylation. Identification of glycopeptides from data-dependent acquisition (DDA) relies on high quality MS/MS spectra of glycopeptide precursors and often… read more here.
Keywords: glycopeptide analysis; data independent; mass spectrometry; analysis ... See more keywords
Targeted data-independent acquisition for mass spectrometric detection of RAS mutations in formalin-fixed, paraffin-embedded tumor biopsies.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2018.04.022
Abstract: Genomic testing for KRAS and NRAS mutations in clinical biopsies of various cancers is routinely performed to predict futility of anti-epidermal growth factor receptor (anti-EGFR) therapies. We hypothesized that RAS mutations could be detected and… read more here.
Keywords: data independent; ras mutations; mass; independent acquisition ... See more keywords
Optimised data-independent acquisition strategy recaptures the classification of early-stage hepatocellular carcinoma based on data-dependent acquisition.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2021.104152
Abstract: Proteomics is increasingly used for exploring disease biomarkers and therapeutic targets. The data-independent acquisition (DIA) method collects all peptide signals in a sample, and provides a convenient way to archive disease-related molecular features for further… read more here.
Keywords: dia; hepatocellular carcinoma; data independent; independent acquisition ... See more keywords
Combinatory data independent acquisition and parallel reaction monitoring method for deep profiling of gangliosides.
Sign Up to like & getrecommendations! Published in 2020 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c02313
Abstract: Ganglioside is an important class of lipid species involving in intercellular signaling and various diseases, especially for neurodegenerative diseases. Systematic ganglioside profiling is challenging due to their naturally low abundance and highly diverse species. Herein,… read more here.
Keywords: data independent; ganglioside species; ganglioside; independent acquisition ... See more keywords
The Isotopic Ac-IP Tag Enables Multiplexed Proteome Quantification in Data-Independent Acquisition Mode
Sign Up to like & getrecommendations! Published in 2021 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.1c00453
Abstract: Data-independent acquisition (DIA) is an increasingly used approach for quantitative proteomics. However, most current isotope labeling strategies are not suitable for DIA as they lead to more complex MS2 spectra or severe ratio distortion. As… read more here.
Keywords: dia; data independent; independent acquisition; tag ... See more keywords