Articles with "indirect elisa" as a keyword



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Establishment of an indirect ELISA method for antibody detection of porcine pseudorabies by recombinant gB, gC, and gD proteins

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Published in 2022 at "Journal of Medical Virology"

DOI: 10.1002/jmv.28228

Abstract: Pseudorabies virus (PRV), as a neuroherpes virus, leads to heavy economic losses in the pig industry worldwide. This study was designed to establish recombinant PRV glycoprotein B (gB), C, and D proteins as PRV diagnostic… read more here.

Keywords: establishment indirect; indirect elisa; recombinant proteins; elisa method ... See more keywords
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Development of an indirect ELISA assay for detecting antibodies against mammalian reovirus in pigs.

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Published in 2018 at "Journal of virological methods"

DOI: 10.1016/j.jviromet.2018.07.008

Abstract: Mammalian reovirus (MRV) infects many species. Over the past decades, MRV infections in pigs have been reported, and several highly pathogenic MRV strains have recently been isolated in the United States. In this study, an… read more here.

Keywords: mammalian reovirus; reovirus; assay detecting; elisa assay ... See more keywords
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Development of an indirect ELISA based on soluble antigen produced from virus-infected cells for detection of Porcine Hemagglutinating Encephalomyelitis virus.

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Published in 2020 at "Journal of virological methods"

DOI: 10.1016/j.jviromet.2020.114016

Abstract: Porcine hemagglutinating encephalomyelitis virus (PHEV) is a member of the genus Betacoronavirus and is the etiologic agent of encephalomyelitis or vomiting and wasting disease in neonatal pigs. Although there are only a few epidemiological studies… read more here.

Keywords: indirect elisa; encephalomyelitis; virus; porcine hemagglutinating ... See more keywords
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Quantitative Detection of Anti-SARS-CoV-2 Antibodies Using Indirect ELISA

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Published in 2021 at "Laboratory Medicine"

DOI: 10.1093/labmed/lmab085

Abstract: Abstract Objective Real-time reverse transcription-polymerase chain reaction is the gold standard for the diagnosis of COVID-19, but it is necessary to utilize other tests to determine the burden of the disease and the spread of… read more here.

Keywords: detection; igg igm; sars cov; igm iga ... See more keywords
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Development of a VP38 recombinant protein-based indirect ELISA for detection of antibodies against grass carp reovirus genotype II (iELISA for detection of antibodies against GCRV II).

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Published in 2018 at "Journal of fish diseases"

DOI: 10.1111/jfd.12890

Abstract: Currently, serological assays for grass carp reovirus genotype II (GCRV-II) diagnosis are not available. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against GCRV-II was developed. The structural protein… read more here.

Keywords: carp reovirus; reovirus genotype; detection antibodies; grass carp ... See more keywords
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Establishment and evaluation of an indirect ELISA for detection of antibodies to goat Klebsiella pneumonia

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Published in 2021 at "BMC Veterinary Research"

DOI: 10.1186/s12917-021-02820-1

Abstract: Background Klebsiella pneumonia , a Gram-negative bacterium belonging to the genus Enterobacter , causes many human and livestock diseases. Notably, infected goats may develop pneumonia, septicemia, which can lead to occasional death, resulting in great… read more here.

Keywords: detection; klebsiella; indirect elisa; klebsiella pneumonia ... See more keywords
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The serodiagnostic potential of recombinant proteins TES–30 and TES–120 in an indirect ELISA in the diagnosis of toxocariasis in cattle, horses, and sheep

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Published in 2019 at "PLoS ONE"

DOI: 10.1371/journal.pone.0213830

Abstract: Toxocariasis is a zoonotic disease that affects humans and animals alike. Although recombinant proteins are widely used for its diagnosis in humans, their performance in companion and production animals remains unknown. This study aimed to… read more here.

Keywords: toxocariasis; serodiagnostic potential; potential recombinant; cattle horses ... See more keywords
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Evaluation of infection with N protein-specific Immunoglobulin M and G in naturally occurring distemper in dogs

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Published in 2020 at "Veterinarni Medicina"

DOI: 10.17221/31/2019-vetmed

Abstract: In dogs, canine distemper has a worldwide distribution with high morbidity/mortality, despite the widespread usage of vaccines and has no specific treatment. In susceptible animals with the canine distemper virus, respiratory, gastrointestinal and nervous system… read more here.

Keywords: infection; canine distemper; protein specific; evaluation ... See more keywords
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Potentials of Glycoprotein Fraction in Successful Diagnosis of Sheep Haemonchosis

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Published in 2020 at "Advances in Animal and Veterinary Sciences"

DOI: 10.17582/journal.aavs/2021/9.3.335.343

Abstract: | Haemonchus contortus (H. contortus) is a pathogenic nematode of small ruminants. In sheep, H. contortus causes anemia that decreases animal productivity and can ultimately lead to death. The objective of the current study was… read more here.

Keywords: indirect elisa; contortus; gluc fraction; fraction ... See more keywords
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Development of an indirect ELISA against African swine fever virus using two recombinant antigens, partial p22 and p30.

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Published in 2022 at "Journal of virological methods"

DOI: 10.2139/ssrn.4147737

Abstract: African swine fever (ASF) is a highly fatal viral disease affecting pigs. It is caused by the ASF virus (ASFV), and causes serious economic losses to the swine industry worldwide, including in Korea. Commercially available… read more here.

Keywords: asfv; indirect elisa; p22 p30; african swine ... See more keywords

Development of an Indirect ELISA Kit for Rapid Detection of Varicella-Zoster Virus Antibody by Glycoprotein E

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Published in 2022 at "Frontiers in Microbiology"

DOI: 10.3389/fmicb.2022.897752

Abstract: Varicella-zoster virus (VZV), a highly infectious agent that causes varicella (chickenpox), can also cause zoster (shingles), a disorder that is frequently associated with severe neuralgia. A reliable serological VZV diagnostic assay would be useful for… read more here.

Keywords: indirect elisa; vzv; zoster; elisa kit ... See more keywords