Co‐staining of KCa3.1 Channels in NSCLC Cells with a Small‐Molecule Fluorescent Probe and Antibody‐Based Indirect Immunofluorescence
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DOI: 10.1002/cmdc.202000652
Abstract: The Ca2+ activated potassium channel 3.1 (KCa3.1) is involved in critical steps of the metastatic cascade, such as proliferation, migration, invasion and extravasation. Therefore, a fast and efficient protocol for imaging of KCa3.1 channels was… read more here.
Keywords: indirect immunofluorescence; antibody; kca3; channel ... See more keywords
Towards the automatic segmentation of HEp-2 cells in indirect immunofluorescence images using an efficient filtering based approach
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DOI: 10.1007/s11042-020-08651-w
Abstract: The computer aided analysis of Indirect-Immunofluorescence (IIF) images is important for the differential diagnosis of several autoimmune diseases. A fully automatic approach consists in segmentation of individual cells in IIF images and subsequently its classification… read more here.
Keywords: filtering based; indirect immunofluorescence; iif images; approach ... See more keywords
Comparison between direct and indirect immunofluorescence method for determination of somatic cell count
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DOI: 10.1007/s11696-018-0445-3
Abstract: A sensitive indirect immunofluorescence (IF) assay for bovine neutrophil and somatic cell counting was developed. The obtained indirect IF was compared with direct IF. The direct IF method uses a single-fluorophore-conjugated antibody directed against the target… read more here.
Keywords: somatic cell; indirect immunofluorescence; antibody; method ... See more keywords
pANCA autoantibody testing by indirect immunofluorescence indicates interstitial arteritis independent of MPO-ANCA immunoassays in ANCA-associated glomerulonephritis
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DOI: 10.1007/s40620-022-01320-1
Abstract: estimated filtration glomerulitis, glomerulonephritis, immunoglobulin G, immunofluorescence, inflammation myeloperoxidase, read more here.
Keywords: testing indirect; immunofluorescence; glomerulonephritis; panca autoantibody ... See more keywords
Combining multi-antigenic immunodot with indirect immunofluorescence on HEp-2 cells improves the diagnosis of systemic sclerosis.
Sign Up to like & getrecommendations! Published in 2021 at "Clinical immunology"
DOI: 10.1016/j.clim.2021.108774
Abstract: Systemic sclerosis (SSc) is associated, in nearly all patients, with autoantibodies (Ab). Accordingly, and in order to identify major (anti-CEN A/B and anti-Topo I) but also minor Abs, the usefulness of combining indirect immunofluorescence (IIF)… read more here.
Keywords: ssc; indirect immunofluorescence; multi antigenic; hep ... See more keywords
Prozone phenomenon observed in indirect immunofluorescence assay by antibodies against neuronal antigens
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DOI: 10.1016/j.jneuroim.2020.577415
Abstract: A marked prozone effect was observed in indirect immunofluorescence with human sera and human cerebrospinal fluid in two clinical cases involving breast carcinoma with paraneoplastic neuronal antibodies, and anti- N-methyl-D-aspartic acid (NMDA) receptor antibodies. Anti-Yo… read more here.
Keywords: indirect immunofluorescence; prozone phenomenon; immunofluorescence; antibodies anti ... See more keywords
Utility of the pattern‐based approach using BIOCHIP‐indirect immunofluorescence in the evaluation of subepidermal bullous disorders
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DOI: 10.1111/cup.14368
Abstract: Subepidermal bullous disorders (SEBD) are a heterogeneous group of vesiculobullous diseases because of antibody‐mediated destruction of proteins of the dermo‐epidermal junction. Direct immunofluorescence (DIF) is the gold standard for diagnosis. BIOCHIP‐indirect immunofluorescence (IIF) is a… read more here.
Keywords: biochip indirect; indirect immunofluorescence; bullous disorders; utility pattern ... See more keywords
Comparison of chemiluminescence microparticle immunoassay, indirect immunofluorescence assay, linear immunoassay and multiple microbead immunoassay detecting autoantibodies in systemic lupus erythematosus
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DOI: 10.1111/sji.12849
Abstract: The aim of study was to detect antinuclear antibodies (ANA) using indirect immunofluorescence assay (IIFA), linear immunoassay (LIA), chemiluminescence microparticle immunoassay (CMIA), multiple microbead immunoassay (MBI) and to compare these four methods in the performance… read more here.
Keywords: linear immunoassay; indirect immunofluorescence; sle; cmia ... See more keywords
Antinuclear antibodies by indirect immunofluorescence and solid phase assays
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DOI: 10.1136/annrheumdis-2019-215443
Abstract: Pisetsky et al recently reported substantial variability between two antinuclear antibodies (ANA) indirect immunofluorescence (IIF) assays as well as one solid-phase assay (SPA) from different manufacturers in a study of a cross-sectional cohort of systemic… read more here.
Keywords: solid phase; iif; indirect immunofluorescence; antinuclear antibodies ... See more keywords
Response to ‘Antinuclear antibodies by indirect immunofluorescence and solid phase assays’ by Bossuyt et al
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DOI: 10.1136/annrheumdis-2019-215479
Abstract: We would like to thank Dr Bossuyt and colleagues1 for their comments on our article on variability in testing for antinuclear antibodies (ANA).2 This letter, along with previous correspondence,3–9 highlights the many issues about ANA… read more here.
Keywords: indirect immunofluorescence; antinuclear antibodies; immunofluorescence solid; antibodies indirect ... See more keywords
Evaluation of a Fully Automated Antinuclear Antibody Indirect Immunofluorescence Assay in Routine Use
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2020.607541
Abstract: Indirect immunofluorescence assay (IFA) using HEp-2 cells as a substrate is the gold standard for detecting antinuclear antibodies (ANA) in patient serum. However, the ANA IFA has labor-intensive nature of the procedure and lacks adequate… read more here.
Keywords: indirect immunofluorescence; fully automated; routine use; immunofluorescence assay ... See more keywords