Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets
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DOI: 10.1021/acs.analchem.6b02528
Abstract: Fluorescence anisotropy measurements of reagents compartmentalized into individual nanoliter droplets are shown to yield high-resolution binding curves from which precise dissociation constants (Kd) for protein–peptide interactions can be inferred. With the current platform, four titrations… read more here.
Keywords: fluorescence; fluorescence anisotropy; anisotropy measurements; individual nanoliter ... See more keywords