DNA Double-Strand Break-Induced Gene Amplification in Yeast.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0644-5_17
Abstract: Precise control of the gene copy number in the model yeast Saccharomyces cerevisiae may facilitate elucidation of enzyme functions or, in cell factory design, can be used to optimize production of proteins and metabolites. Currently,… read more here.
Keywords: induced gene; dna double; strand break; double strand ... See more keywords
Virus-Induced Gene Silencing (VIGS) in Flax (Linum usitatissimum L.) Seed Coat: Description of an Effective Procedure Using the transparent testa 2 Gene as a Selectable Marker.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0751-0_17
Abstract: Virus-induced gene silencing (VIGS) has been successfully applied for functional analysis of genes in many plant species. Many protocols have been established but mainly for gene expression study in vegetative tissue. Here, we present the… read more here.
Keywords: induced gene; seed coat; seed; virus induced ... See more keywords
Virus-Induced Gene Silencing of Cell Wall Genes in Flax (Linum usitatissimum).
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0751-0_6
Abstract: Plants have developed defense mechanisms against viruses by using an RNA silencing-based process, which has many common features with the endogenous RNA silencing pathway used for regulating the level of transcripts derived from developmental genes.… read more here.
Keywords: induced gene; virus induced; gene silencing; gene ... See more keywords
Host-Induced Gene Silencing (HIGS) for Elucidating Puccinia Gene Function in Wheat.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8724-5_12
Abstract: Biotrophic fungi (Puccinia spp.) cause devastating diseases of wheat and other cereal species globally. The function of large repertories of genes from Puccinia spp. still needs to be discovered to understand the infection process of… read more here.
Keywords: induced gene; silencing higs; host induced; gene function ... See more keywords
Confounding influence of tamoxifen in mouse models of Cre recombinase-induced gene activity or modulation
Sign Up to like & getrecommendations! Published in 2018 at "Archives of Toxicology"
DOI: 10.1007/s00204-018-2254-4
Abstract: Tamoxifen (TAM) is commonly used for cell type specific Cre recombinase-induced gene inactivation and in cell fate tracing studies. Inducing a gene knockout by TAM and using non-TAM exposed mice as controls lead to a… read more here.
Keywords: induced gene; tamoxifen; recombinase induced; cre recombinase ... See more keywords
Advanced strategies to control plant pathogenic fungi by host-induced gene silencing (HIGS) and spray-induced gene silencing (SIGS)
Sign Up to like & getrecommendations! Published in 2019 at "Plant Biotechnology Reports"
DOI: 10.1007/s11816-019-00588-3
Abstract: Plant pathogenic fungi and oomycetes cause severe losses of crop yield worldwide. Fungicides are widely applied to manage plant diseases caused by pathogenic fungi, but fungicide-resistant fungal populations have been increasingly reported. Recent techniques using… read more here.
Keywords: induced gene; spray; plant; pathogenic fungi ... See more keywords
Virus-induced Gene Silencing in Streptocarpus rexii (Gesneriaceae)
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Biotechnology"
DOI: 10.1007/s12033-020-00248-w
Abstract: Many members of the family Gesneriaceae are cultivated as ornamental plants, including Cape primrose (Streptocarpus) species. The range of plant architecture found in this genus has also made it a model to study leaf and… read more here.
Keywords: induced gene; streptocarpus rexii; virus induced; gene silencing ... See more keywords
Host-induced gene silencing of the acetolactate synthases VdILV2 and VdILV6 confers resistance to Verticillium wilt in cotton (Gossypium hirsutum L.).
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2020.01.126
Abstract: Cotton Verticillium wilt caused by Verticillium dahliae (V. dahliae) is one of the most destructive fungal diseases and is difficult to control. However, resistant germplasm resources are scarce in cotton. Many studies have shown that… read more here.
Keywords: verticillium; induced gene; verticillium wilt; host induced ... See more keywords
Exposure to polystyrene microplastics induced gene modulated biological responses in zebrafish (Danio rerio).
Sign Up to like & getrecommendations! Published in 2020 at "Chemosphere"
DOI: 10.1016/j.chemosphere.2020.128592
Abstract: The substantial increase in the occurrence of microplastics (MPs) in the aquatic ecosystem has been recognized as an emerging concern today. Studies have revealed the toxicity of microplastics on behavior, physiology, and reproduction of fishes.… read more here.
Keywords: induced gene; exposure polystyrene; microplastics induced; polystyrene microplastics ... See more keywords
Identification and characterization of pectin related gene NbGAE6 through virus-induced gene silencing in Nicotiana benthamiana.
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DOI: 10.1016/j.gene.2020.144522
Abstract: Virus-induced gene silencing (VIGS) is a transient based reverse genetic tool used to elucidate the function of novel gene in N.benthamiana. . In current study, 14 UDP-D-glucuronate 4-epimerase (GAE) family members were identified and their… read more here.
Keywords: induced gene; pectin; virus induced; gene silencing ... See more keywords
Towards mastering CRISPR-induced gene knock-in in plants: Survey of key features and focus on the model Physcomitrella patens.
Sign Up to like & getrecommendations! Published in 2017 at "Methods"
DOI: 10.1016/j.ymeth.2017.04.024
Abstract: Beyond its predominant role in human and animal therapy, the CRISPR-Cas9 system has also become an essential tool for plant research and plant breeding. Agronomic applications rely on the mastery of gene inactivation and gene… read more here.
Keywords: knock; induced gene; crispr; physcomitrella patens ... See more keywords