Light-Inducible CRISPR Labeling.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0755-8_9
Abstract: CRISPR labeling is a powerful technique to study the chromatin architecture in live cells. In CRISPR labeling, a catalytically dead CRISPR-Cas9 mutant is employed as programmable DNA-binding domain to recruit fluorescent proteins to selected genomic… read more here.
Keywords: light inducible; crispr; microscopy; inducible crispr ... See more keywords
Drug Inducible CRISPR/Cas Systems
Sign Up to like & getrecommendations! Published in 2019 at "Computational and Structural Biotechnology Journal"
DOI: 10.1016/j.csbj.2019.07.015
Abstract: Clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) systems have been employed as a powerful versatile technology for programmable gene editing, transcriptional modulation, epigenetic modulation, and genome labeling, etc. Yet better control of their… read more here.
Keywords: inducible crispr; crispr cas; drug inducible; cas ... See more keywords
Small-Molecule-Mediated Split-Aptamer Assembly for Inducible CRISPR-dCas9 Transcription Activation.
Sign Up to like & getrecommendations! Published in 2022 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00101
Abstract: Inducible CRISPR-dCas9 transcription system has become a powerful tool for transcription regulation and sensing. Here, we develop a new concept of small-molecule-mediated split-aptamer assembly for inducible CRISPR-dCas9 transcription activation, allowing quantitative detection and imaging of… read more here.
Keywords: inducible crispr; dcas9 transcription; transcription; dcas9 ... See more keywords