Generation of a dual edited human induced pluripotent stem cell Myl7-GFP reporter line with inducible CRISPRi/dCas9.
Sign Up to like & getrecommendations! Published in 2022 at "Stem cell research"
DOI: 10.1016/j.scr.2022.102754
Abstract: Temporal regulation of CRISPRi activity is critical for genetic screens. Here, we present an inducible CRISPRi platform enabling selection of iPSC-derived cardiomyocytes and reversible gene knockdown. We targeted a doxycycline-inducible dCas9-KRAB-mCherry cassette into the AAVS1… read more here.
Keywords: myl7; dcas9; ipsc; stem cell ... See more keywords
A Leak-Free Inducible CRISPRi/a System for Gene Functional Studies in Plasmodium falciparum
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DOI: 10.1128/spectrum.02782-21
Abstract: Understanding the fundamental biology of malaria parasites through functional genetic/genomic studies is critical for identifying novel targets for antimalarial development. Conditional knockout/knockdown systems are required to study essential genes in the haploid blood stages of… read more here.
Keywords: system; crispri system; crispri; inducible crispri ... See more keywords
Generation and validation of versatile inducible CRISPRi embryonic stem cell and mouse model
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DOI: 10.1371/journal.pbio.3000749
Abstract: Clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated (Cas) 9 has been widely used far beyond genome editing. Fusions of deactivated Cas9 (dCas9) to transcription effectors enable interrogation of the epigenome and controlling of gene expression.… read more here.
Keywords: inducible crispri; crispri; expression; embryonic stem ... See more keywords