Establishment of a low‐dosage‐IPTG inducible expression system construction method in Escherichia coli
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.201800160
Abstract: The lac operon is a delicate inducible gene expression element in bacteria. To efficiently induce gene expression, a sufficient dosage of an inducer, usually that of 500–1000 µM isopropyl β‐D‐1‐thiogalactopyranoside (IPTG), is required to keep repressor… read more here.
Keywords: expression; iptg inducible; dosage iptg; inducible expression ... See more keywords
Design of a Lentiviral Vector for the Inducible Expression of MYC: A New Strategy for Construction Approach
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Biotechnology"
DOI: 10.1007/s12033-017-0006-y
Abstract: Lentiviral vectors are powerful tools for gene expression studies. Here we report the construction of pTIJ, a vector for inducible gene expression. pTIJ was generated from pTRIPZ backbone, which is designed for the inducible expression… read more here.
Keywords: vector inducible; construction; ptij; expression ... See more keywords
Enhanced growth-driven stepwise inducible expression system development in haloalkaliphilic desulfurizing Thioalkalivibrio versutus.
Sign Up to like & getrecommendations! Published in 2019 at "Bioresource technology"
DOI: 10.1016/j.biortech.2019.121486
Abstract: Highly toxic and flammable H2S gas has become an environmental threat. Because of its ability to efficiently remove H2S by oxidation, Thioalkalivibrio versutus is gaining more attention. Haloalkaliphilic autotrophs, like the bio-desulfurizing T. versutus, grow… read more here.
Keywords: thioalkalivibrio versutus; system; versutus; inducible expression ... See more keywords
Generation of a human embryonic stem cell (WAe001-A-47) with hVGLL4 doxycyclin-inducible expression by the PiggyBac transposon system.
Sign Up to like & getrecommendations! Published in 2020 at "Stem cell research"
DOI: 10.1016/j.scr.2020.102142
Abstract: VGLL4 is a new component of the Hippo pathway and bind TEADs to compete with YAP, so as to inhibit tumor progression, but its role in stem cell and organ regeneration remains unclear. Using the… read more here.
Keywords: piggybac transposon; transposon system; stem cell; stem ... See more keywords
Stepwise Optimization of Inducible Expression System for the Functional Secretion of Horseradish Peroxidase in Saccharomyces cerevisiae.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.2c09117
Abstract: Horseradish peroxidase (HRP) is a plant-derived glycoprotein that can be developed as a food additive to cross-link proteins or biopolymers. Although Saccharomyces cerevisiae has advantages in the production of food-grade HRP, the low expressional level… read more here.
Keywords: horseradish peroxidase; hrp; inducible expression; expression ... See more keywords
Copper-inducible expression system for metabolic engineering of Escherichia coli.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of applied microbiology"
DOI: 10.1093/jambio/lxad109
Abstract: AIMS The inducible expression system plays an important role in engineering Escherichia coli for chemical production. However, it still heavily relies on expensive chemical inducers, like IPTG. There is a pressing need to develop alternative… read more here.
Keywords: system; expression system; inducible expression; expression ... See more keywords
Membrane Fluidization Governs the Coordinated Heat-Inducible Expression of Nucleus- and Plastid Genome-Encoded Heat Shock Protein 70 Genes in the Marine Red Alga Neopyropia yezoensis
Sign Up to like & getrecommendations! Published in 2023 at "Plants"
DOI: 10.3390/plants12112070
Abstract: Heat shock protein 70 (HSP70) is an evolutionarily conserved protein chaperone in prokaryotic and eukaryotic organisms. This family is involved in the maintenance of physiological homeostasis by ensuring the proper folding and refolding of proteins.… read more here.
Keywords: heat inducible; inducible expression; expression; heat ... See more keywords