Grad-cryo-EM: Tool to Isolate Translation Initiation Complexes from Rabbit Reticulocyte Lysate Suitable for Structural Studies.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0278-2_21
Abstract: Since its development, single-particle cryogenic electron microscopy (cryo-EM) has played a central role in the study at medium resolution of both bacterial and eukaryotic ribosomal complexes. With the advent of the direct electron detectors and… read more here.
Keywords: initiation complexes; initiation; rabbit reticulocyte; reticulocyte lysate ... See more keywords
Structural Insights into the Mammalian Late-Stage Initiation Complexes
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DOI: 10.1016/j.celrep.2020.03.061
Abstract: Summary In higher eukaryotes, the mRNA sequence in the direct vicinity of the start codon, called the Kozak sequence (CRCCaugG, where R is a purine), is known to influence the rate of the initiation process.… read more here.
Keywords: late stage; initiation complexes; initiation; structural insights ... See more keywords
Assembly and Dynamics of Transcription Initiation Complexes.
Sign Up to like & getrecommendations! Published in 2025 at "Annual review of biochemistry"
DOI: 10.1146/annurev-biochem-072324-035226
Abstract: Gene expression is essential for life and development, allowing the cell to modulate mRNA production in response to intrinsic and extracellular cues. Initiation of gene transcription requires a highly regulated molecular process to assemble multisubunit… read more here.
Keywords: initiation complexes; dynamics transcription; initiation; transcription initiation ... See more keywords