Impact of dual baculovirus infection on the Sf9 insect cell transcriptome during AAV production using single-cell RNA-seq.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28377
Abstract: The insect cell-baculovirus expression vector system (IC-BEVS) has shown to be a powerful platform to produce complex biopharmaceutical products, such as recombinant proteins and virus-like particles (VLPs). More recently IC-BEVS has been also used as… read more here.
Keywords: cell; insect cell; sf9 insect; dual baculovirus ... See more keywords
Insect cell culture as a tool in plant virus research: a historical overview
Sign Up to like & getrecommendations! Published in 2020 at "Phytoparasitica"
DOI: 10.1007/s12600-020-00795-7
Abstract: Culturing insect cells in vitro has witnessed remarkable growth in the past decades. Over 600 cell lines of dipteran, hemipteran, and lepidopteran insects have been reported so far. Besides its wide adoption for the production… read more here.
Keywords: cell culture; plant virus; insect cell; plant ... See more keywords
Development of insect cell line using CRISPR technology.
Sign Up to like & getrecommendations! Published in 2021 at "Progress in molecular biology and translational science"
DOI: 10.1016/bs.pmbts.2021.01.003
Abstract: In this chapter, we delineated the methods of CRISPR technology that has been used for the development of engineered insect cell line. We elaborated on how CRISPR/Cas9 genome editing in Drosophila melanogaster, Bombyx mori, Spodoptera… read more here.
Keywords: cell line; crispr technology; insect cell; cell ... See more keywords
Human and insect cell-produced rAAVs show differences in genome heterogeneity.
Sign Up to like & getrecommendations! Published in 2022 at "Human gene therapy"
DOI: 10.1089/hum.2022.050
Abstract: In the past two decades, adeno-associated virus (AAV) vector manufacturing has made remarkable advancements to meet large-scale production demands for pre-clinical and clinical trials. In addition, AAV vectors have been extensively studied for their safety… read more here.
Keywords: insect cell; cell produced; produced raavs; raavs show ... See more keywords
Insect Cell-Based Models: Cell Line Establishment and Application in Insecticide Screening and Toxicology Research
Sign Up to like & getrecommendations! Published in 2023 at "Insects"
DOI: 10.3390/insects14020104
Abstract: Simple Summary Insect cell lines have often been employed in pest management, where they have been used as tools to evaluate the activity and explore the toxic mechanisms of insecticide candidate compounds. This review summarizes… read more here.
Keywords: toxicology; insect cell; cell; cell line ... See more keywords
Improved Expression of SARS-CoV-2 Spike RBD Using the Insect Cell-Baculovirus System
Sign Up to like & getrecommendations! Published in 2022 at "Viruses"
DOI: 10.3390/v14122794
Abstract: Insect cell-baculovirus expression vector system is one of the most established platforms to produce biological products, and it plays a fundamental role in the context of COVID-19 emergency, providing recombinant proteins for treatment, diagnosis, and… read more here.
Keywords: cell; insect cell; cell baculovirus; rbd ... See more keywords
Innovations in the Insect Cell Expression System for Industrial Recombinant Vaccine Antigen Production
Sign Up to like & getrecommendations! Published in 2021 at "Vaccines"
DOI: 10.3390/vaccines9121504
Abstract: The insect cell expression system has previously been proposed as the preferred biosecurity strategy for production of any vaccine, particularly for future influenza pandemic vaccines. The development and regulatory risk for new vaccine candidates is… read more here.
Keywords: expression system; vaccine; production; cell expression ... See more keywords